刘元东-CSCIED科技核心评价数据库-手机版

单位：中南大学

研究方向：生物技术（矿产资源处理与环境修复方向）

社会任职
工作于中南大学资源加工与生物工程学院和生物冶金教育部重点实验室，长期从事生物冶金与极端环境微生物、矿物资源处理及其污染治理、生物材料与抗菌材料、生物传感器与环境监测、蛋白质功能结构解析与分子生物学、多层生物组学、生物信息学与软件开发等的研究，是教育部和科技部评审专家、湖南省和广东省科技评审专家、长沙市科技评审专家，国际生物湿法冶金学会副秘书、Frontiers in Bioscience-Landmark、Separation、Frontiers in Bioleaching等国际杂志审稿人。主持或参与国家级省部级等项目10多项，发表SCI论文60多篇，获得专利30多项，获得软件著作权20多项，获得国际及省级等奖励荣誉4项。

个人介绍
国家科技部和自然科学基金委评审专家教育部科技和学位评审专家湖南省和广东省科技评审专家长沙市科技评审专家国际生物湿法冶金学会副秘书 “生物冶金与矿冶环境治理丛书”编委 Frontiers in Bioscience-Landmark, Separation, Frontiers in Bioleaching 等国际杂志编委及审稿人

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1. Isolation,Sphalerite Bioleaching,and Whole Genome Sequencing of Acidithiobacillus ferriphilus QBS3 from Zinc-rich Sulfide Mine Drainage

[学者论文] RS评分：0

作者： Kan Wang, Yuandong Liu*, Run Liu, Wissal Belqadi, Weimin Zeng, Runlan Yu,Xueling Wu

《Life》2025年 15卷 5期

摘要：The genus Acidithiobacillus has been widely used in bioleaching, and novel strains in this genus, such as A. ferriphilus, have also been conffrmed to possess bioleaching capabilities. In this study, an Acidithiobacillus ferriphilus strain, QBS3, was isolated from zincrich sulffde mine drainage using the gradient dilution method. QBS3 is a Gram-negative, 1.3 µm rod-shaped bacterium with small red colonies. It showed a high iron oxidation efffciency of 0.361 g/(L·h) and a sulfur oxidation efffciency of 0.206 g/(L·d). QBS3 has sphalerite bioleaching ability; using QBS3 for pure sphalerite bioleaching, 18.8% of zinc was extracted in 14 days at 1% pulp density. Whole genome sequencing was performed on QBS3. Functional prediction showed that 9.13% of the genes were involved in replication, recombination, and repair. Bioleaching-related genes were analyzed, including iron and sulfur oxidation genes, and carbon and nitrogen ffxation genes. For iron oxidation, the Cyc2→RusA pathway and Iro→RusB pathway were found in QBS3. In terms of sulfur oxidation, QBS3 has an incomplete SOX system and lacks the SDO gene, but Rho and Trx may complement the SOX system, enabling QBS3 to oxidize sulfur. QBS3 has multiple sets of carbon ffxation genes, and nitrogen ffxation genes were also identiffed. A hypothetical sphalerite bioleaching model is proposed; this study provides a theoretical basis for the zinc sulffde ore bioleaching industry.

关键词：Acidithiobacillus ferriphilus; isolation; bioleaching; sphalerite; ferrous oxidation; sulfur oxidation

浏览量: 64 下载: 0 发表时间：2025/5/15 0:00:00

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2. Expression, Purification and Characterization of a Novel Rusticyanin from the Psychrotolerant Acidithiobacillus ferrivorans

[学者论文] RS评分：0

作者： Yuandong Liu*, Lixiang Chen, Xiangdong Shangguan, Jingying Ouyang, Jiayu He, Kan Wang, Yan Tong,Runlan Yu, Weimin Zeng, Xueling Wu, Li Shen,Guanzhou Qiu

《Separations》2023年 10卷 8期

摘要：Rusticyanin plays a crucial role in ferrous oxidation of sulﬁde minerals during bioleaching for industrial metal extraction. Diverse isoforms of rusticyanin have been found, but until now, except for type-A rusticyanin, other isoforms or sources of rusticyanin have been scarcely investigated. Here, a rusticyanin (gene locus 0470) from the psychrophilic Acidithiobacillus ferrivorans was gene-cloned, expressed, puriﬁed, and assembled in vitro. All forms of the protein exhibit extreme acid stability, even at pH 0.3. The stability of the protein is obviously enhanced after binding of the copper cofactor; the oxidation state is more stable than the reduced state. The protein has characteristic UV-vis peaks and EPR signals similar to type-A or type-B rusticyanin but is different with a small position shift and an obvious intensity change. The vibrational spectrum of the apoprotein was more different than these between the oxidation and reduced states of the protein. The ferrous oxidation kinetic rate constant of the protein is obviously faster than that of both the type-A and type-B rusticyanins previously reported. Further bioinformatics analysis reveal their changes in sequence and molecule: the mutations related to the peculiar shield belt mostly account for the variation in the properties of the protein, and the classiﬁcation of the protein as a new isoform, type-C rusticyanin, is proposed.

关键词：rusticyanin; isoform; Acidithiobacillus ferrivorans; overexpression; characterization; kinetic rate constant

浏览量: 64 下载: 0 发表时间：2023/11/17 0:00:00

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3. Silicon Nitride Bioceramics Sintered by Microwave Exhibit Excellent Mechanical Properties, Cytocompatibility In Vitro,and Anti-Bacterial Properties

[学者论文] RS评分：0

作者： Jiayu He, Yuandong Liu*, Xiaofeng Zeng, Yan Tong, Run Liu, Kan Wang, Xiangdong Shangguan, Guanzhou Qiu,Coswald Stephen Sipaut

《Journal of Functional Biomaterials》2023年 14卷 11期

摘要：Silicon nitride is a bioceramic with great potential, and multiple studies have demonstrated its biocompatibility and antibacterial properties. In this study, silicon nitride was prepared by a microwave sintering technique that was different from common production methods. SEM and pore distribution analysis revealed the microstructure of microwave-sintered silicon nitride with obvious pores. Mechanical performance analysis shows that microwave sintering can improve the mechanical properties of silicon nitride. The CCK-8 method was used to demonstrate that microwave-sintered silicon nitride has no cytotoxicity and good cytocompatibility. From SEM and CLSM observations, it was observed that there was good adhesion and cross-linking of cells during microwave-sintered silicon nitride, and the morphology of the cytoskeleton was good. Microwave-sintered silicon nitride has been proven to be non-cytotoxic. In addition, the antibacterial ability of microwavesintered silicon nitride against Staphylococcus aureus and Escherichia coli was tested, proving that it has a good antibacterial ability similar to the silicon nitride prepared by commonly used processes. Compared with silicon nitride prepared by gas pressure sintering technology, microwave-sintered silicon nitride has excellent performance in mechanical properties, cell compatibility, and antibacterial properties. This indicates its enormous potential as a substitute material for manufacturing bone implants.

关键词：silicon nitride; microwave sintering; mechanical properties; cytocompatibility; anti-bacteria

浏览量: 65 下载: 0 发表时间：2023/11/17 0:00:00

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1. Isolation,Sphalerite Bioleaching,and Whole Genome Sequencing of Acidithiobacillus ferriphilus QBS3 from Zinc-rich Sulfide Mine Drainage

2. Expression, Purification and Characterization of a Novel Rusticyanin from the Psychrotolerant Acidithiobacillus ferrivorans

3. Silicon Nitride Bioceramics Sintered by Microwave Exhibit Excellent Mechanical Properties, Cytocompatibility In Vitro,and Anti-Bacterial Properties