Isolation,Sphalerite Bioleaching,and Whole Genome Sequencing of Acidithiobacillus ferriphilus QBS3 from Zinc-rich Sulfide Mine Drainage-CSCIED科技核心评价数据库-手机版

Isolation,Sphalerite Bioleaching,and Whole Genome Sequencing of Acidithiobacillus ferriphilus QBS3 from Zinc-rich Sulfide Mine Drainage

Isolation,Sphalerite Bioleaching,and Whole Genome Sequencing of Acidithiobacillus ferriphilus QBS3 from Zinc-rich Sulfide Mine Drainage

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DOI	doi.org/10.3390/life15050792
刊名	Life
年，卷(期)	2025, 15(5)
作者	Kan Wang, Yuandong Liu*, Run Liu, Wissal Belqadi, Weimin Zeng, Runlan Yu,Xueling Wu,
作者单位	中南大学
摘要	The genus Acidithiobacillus has been widely used in bioleaching, and novel strains in this genus, such as A. ferriphilus, have also been conffrmed to possess bioleaching capabilities. In this study, an Acidithiobacillus ferriphilus strain, QBS3, was isolated from zincrich sulffde mine drainage using the gradient dilution method. QBS3 is a Gram-negative, 1.3 µm rod-shaped bacterium with small red colonies. It showed a high iron oxidation efffciency of 0.361 g/(L·h) and a sulfur oxidation efffciency of 0.206 g/(L·d). QBS3 has sphalerite bioleaching ability; using QBS3 for pure sphalerite bioleaching, 18.8% of zinc was extracted in 14 days at 1% pulp density. Whole genome sequencing was performed on QBS3. Functional prediction showed that 9.13% of the genes were involved in replication, recombination, and repair. Bioleaching-related genes were analyzed, including iron and sulfur oxidation genes, and carbon and nitrogen ffxation genes. For iron oxidation, the Cyc2→RusA pathway and Iro→RusB pathway were found in QBS3. In terms of sulfur oxidation, QBS3 has an incomplete SOX system and lacks the SDO gene, but Rho and Trx may complement the SOX system, enabling QBS3 to oxidize sulfur. QBS3 has multiple sets of carbon ffxation genes, and nitrogen ffxation genes were also identiffed. A hypothetical sphalerite bioleaching model is proposed; this study provides a theoretical basis for the zinc sulffde ore bioleaching industry.
Abstract	The genus Acidithiobacillus has been widely used in bioleaching, and novel strains in this genus, such as A. ferriphilus, have also been conffrmed to possess bioleaching capabilities. In this study, an Acidithiobacillus ferriphilus strain, QBS3, was isolated from zincrich sulffde mine drainage using the gradient dilution method. QBS3 is a Gram-negative, 1.3 µm rod-shaped bacterium with small red colonies. It showed a high iron oxidation efffciency of 0.361 g/(L·h) and a sulfur oxidation efffciency of 0.206 g/(L·d). QBS3 has sphalerite bioleaching ability; using QBS3 for pure sphalerite bioleaching, 18.8% of zinc was extracted in 14 days at 1% pulp density. Whole genome sequencing was performed on QBS3. Functional prediction showed that 9.13% of the genes were involved in replication, recombination, and repair. Bioleaching-related genes were analyzed, including iron and sulfur oxidation genes, and carbon and nitrogen ffxation genes. For iron oxidation, the Cyc2→RusA pathway and Iro→RusB pathway were found in QBS3. In terms of sulfur oxidation, QBS3 has an incomplete SOX system and lacks the SDO gene, but Rho and Trx may complement the SOX system, enabling QBS3 to oxidize sulfur. QBS3 has multiple sets of carbon ffxation genes, and nitrogen ffxation genes were also identiffed. A hypothetical sphalerite bioleaching model is proposed; this study provides a theoretical basis for the zinc sulffde ore bioleaching industry.
关键词	Acidithiobacillus ferriphilus; isolation; bioleaching; sphalerite; ferrous oxidation; sulfur oxidation
KeyWord	Acidithiobacillus ferriphilus; isolation; bioleaching; sphalerite; ferrous oxidation; sulfur oxidation
基金项目
页码	1-20

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Kan Wang, Yuandong Liu*, Run Liu, Wissal Belqadi, Weimin Zeng, Runlan Yu,Xueling Wu. Isolation,Sphalerite Bioleaching,and Whole Genome Sequencing of Acidithiobacillus ferriphilus QBS3 from Zinc-rich Sulfide Mine Drainage [J]. Life. 2025; 15; (5). 1 - 20.

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