Journal of International Psychiatry

【Objective】To investigate the role of miRNA-132 in in vivo and in vitro epilepsy models.【Methods】Constructing rat model of temporal lobe epilepsy and control by using lithium and pilocarpine, the expression of miRNA was detected by real-time; Screening the regulatory miRNA of SCN2A through bioinformatics analysis; Retinoic acid (RA) was used to construct epilepsy cell model in vitro; the mRNA and protein expression level of miR-132-3p and SCN2A were measured by Real-time PCR, Western blot and immunofluorescence respectively; the mRNA and protein expression of SCN2A was measured in miR-132-3p inhititor treated cells; analysis of the correlation between the expression of miR-132-3p and SCN2A in vivo.【Results】The expression of miR-23a, miR-146a, miR-132, miR-142, miR-199 and miR-34a was significantly increased in rat model of epilepsy; miR-132-3p was the regulatory miRNA of SCN2A;, the mRNA and protein expression of miR-132-3p was significantly increased, while the mRNA and protein expression of SCN2A was decreased significantly in in vitro model of epilepsy; miR-132-3p inhititor induced the expression of SCN2A in in vitro cell model of epilepsy; the expression of miR-132-3p was negatively related to SCN2A.【Conclusion】The expression of miR-132 was increased and the expression of its target gene SCN2A was decreased in in vivo and in vitro epilepsy models. The expression of miR-132-3p was negatively related to SCN2A, suggesting that miR-132 taken part in the development of epilepsy through its target gene SCN2A.

epilepsy; rat model; miRNA; SCN2A