International Journal of Medicine and Data

Objective To study the molecular mechanism of long non coding RNA (LncRNA) regulating meiotic chromosome axis assembly in rabbit spermatogenesis through phase separation. Methods healthy male New Zealand white rabbits were used to carry out the study. Testicular samples were collected for LncRNA screening, identification, phase separation ability verification, chromosome axis assembly detection, etc. Results a total of 2847 LncRNA were identified at each stage of meiosis in rabbits. Among the LncRNA that met the differential expression criteria, there were differences in the stage of meiosis, the number of LncRNA, the specific high expression of LncRNA, and the co expression of chromosome axis genes. In LncRNA-XY1 knockdown test, the SYCP3 axis length of rabbit spermatocytes was significantly shortened, the proportion of complete SC and homologous chromosome pairing rate were significantly reduced, and the meiotic arrest rate was significantly increased. Compared with normal rabbits, the sperm deformity rate of LncRNA knockout rabbits was significantly increased, and the proportion of forward motile sperm, average sperm path velocity and acrosome reaction rate were significantly decreased. Conclusion the loss of LncRNA expression can lead to abnormal assembly of chromosome axis, meiosis block, and the increase of sperm deformity rate and the decrease of sperm motility.

Long non coding RNA; Rabbit; Sperm; Phase separation; Meiosis; Chromosome axis assembly