International Journal of Clinical Research

Objective To explore the distribution of pathogenic bacteria in sputum cultures and the monitoring of drug resistance in ICU patients. Methods From January 2024 to December 2024, 127 patients with severe pneumonia in our ICU were selected. All 127 patients underwent sputum culture. The pathogenic characteristics were analyzed using the Diir D2 mini bacterial identification instrument. The drug sensitivity results were explored using the identification card for drug sensitivity (the drug sensitivity test used the MIC method, and this drug sensitivity card did not contain aztreonam or cefotaxime). Results Among the 127 patients, 238 pathogenic bacteria were detected, including 186 Gram-negative bacteria (78.15%) and 52 Gram-positive bacteria (21.85%). Analysis showed that the resistance rate of Acinetobacter baumannii was the highest to cefazolin, reaching 51.43%; the probability of tolerance to imipenem was relatively low; the resistance rate of Pseudomonas aeruginosa to cefazolin was the highest, reaching 100.00%, and it had no resistance to trimethoprim/ sulfamethoxazole or ampicillin/sulbactam; the resistance rate of Klebsiella pneumoniae to trimethoprim/ sulfamethoxazole, ampicillin/sulbactam, and cefazolin was relatively high. The resistance performance of Staphylococcus aureus to erythromycin was relatively high, reaching 60.87%. Conclusion When selecting antibiotics for ICU patients with severe pneumonia, it is necessary to strengthen management. The use of the Diir D2 mini bacterial identification instrument and the MIC method for drug sensitivity testing (the drug sensitivity card does not contain aztreonam or cefotaxime) is reasonable and scientific, reducing the occurrence of drug-resistant strains and improving the therapeutic effect.

Drug resistance; Sputum specimen; ICU; Pathogenic bacteria; Diir D2 mini bacterial identification instrument; MIC method