基于网络药理学及分子对接探讨蒙药清咽六味散治疗急性咽炎的“活性成分－核心靶点－通路”机制-国际临床研究杂志-CSCIED科技核心评价数据库-手机版

基于网络药理学及分子对接探讨蒙药清咽六味散治疗急性咽炎的“活性成分－核心靶点－通路”机制

Exploring the "active ingredient core target pathway" mechanism of Mongolian medicine Qingyan Liuwei San in the treatment of acute pharyngitis based on network pharmacology and molecular docking

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DOI	10.12208/j.ijcr.20260175
刊名	国际临床研究杂志 International Journal of Clinical Research
年，卷(期)	2026, 10(4)
作者	呼思乐¹, 金媛媛², 斯琴图雅², 吴哈达¹,
作者单位	内蒙古自治区国际蒙医医院内蒙古呼和浩特;
摘要	目的旨在运用网络药理学和分子对接技术，深入探究蒙药经典方剂清咽六味散（QYLWS）在治疗急性咽炎中的药效物质基础及其作用机制。方法利用传统中药系统药理学数据库（TCMSP）及文献资料，筛选出 QYLWS中的主要有效活性成分及其作用靶点；通过GeneCards和OMIM数据库收集急性咽炎相关的疾病靶点，并运用Venny 2.1工具确定药物与疾病的共有靶点；利用STRING数据库进行蛋白质相互作用分析，构建PPI网络，并进行拓扑分析以识别关键靶点。同时，使用Cytoscape 3.10.3软件构建药物－活性成分－靶点网络；采用DAVID数据库对共有靶点进行GO（Gene Ontology）和KEGG（Kyoto Encyclopedia of Genes and Genomes）富集分析，以揭示QYLWS在治疗急性咽炎过程中的生物学过程、分子功能、细胞组分及KEGG信号通路，并通过AutoDockTools软件进行分子对接验证。结果筛选出 QYLWS治疗急性咽炎的槲皮素、柚皮素、苯海拉明等3个核心成分，核心靶点AKT1、IL6、TP53、TNF、CASP3等10个，关键通路涉及癌症途径、脂质与动脉粥样硬化、PI3K-Akt信号通路等20个。分子对接结果亦证实，关键靶点蛋白与核心活性成分之间具有良好的结合活性。结论本研究初步阐明了QYLWS通过多成分、多靶点、多通路的复杂机制发挥其抗感染治疗作用，为QYLWS的临床应用及深入研究提供了坚实的科学依据。
Abstract	Objective To explore the pharmacological substance basis and mechanism of action of the classic Mongolian medicine formula Qingyan Liuwei San in the treatment of acute pharyngitis using network pharmacology and molecular docking technology. Methods Using the Traditional Chinese Medicine Systems Pharmacology Database (TCMSP) and literature data, the main active ingredients and their targets in Qingyan Liuwei San were screened; Collect disease targets related to acute pharyngitis through GeneCards and OMIM databases, and use Venny 2.1 tool to identify common targets between drugs and diseases; Using STRING database for protein interaction analysis, constructing PPI network, and conducting topological analysis to identify key targets. Meanwhile, Cytoscape 3.10.3 software was used to construct a drug active ingredient target network; Using the DAVID database, GO(Gene Ontology) and KEGG(Kyoto Encyclopedia of Genes and Genomes) enrichment analysis were performed on common targets to reveal the biological processes, molecular functions, cellular components, and KEGG signaling pathways of Qingyan Liuwei San in the treatment of acute pharyngitis. Molecular docking validation was performed using AutoDockTools software. Results Three core components, including quercetin, naringenin and diphenhydramine, were screened out from Qingyan Liuwei Powder for the treatment of acute pharyngitis, 10 core targets, including AKT1, IL6, TP53, TNF and CASP3, and 20 key pathways, including cancer pathway, lipid and atherosclerosis, and PI3K Akt signaling pathway. The molecular docking results also confirmed that there is good binding activity between the key target protein and the core active ingredient. Conclusion This study preliminarily elucidated the anti-inflammatory therapeutic effect of Qingyan Liuwei San through a complex mechanism of multiple components, targets, and pathways, providing a solid scientific basis for the clinical application and in-depth research of Qingyan Liuwei San.
关键词	急性咽炎；清咽六味散；网络药理学；药效物质；作用机制
KeyWord	Acute pharyngitis; Qingyan Liuwei San; Network pharmacology; Pharmacological substance; Mechanism of action
基金项目
页码	22-32

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呼思乐, 金媛媛, 斯琴图雅, 吴哈达. 基于网络药理学及分子对接探讨蒙药清咽六味散治疗急性咽炎的“活性成分－核心靶点－通路”机制 [J]. 国际临床研究杂志. 2026; 10; (4). 22 - 32.

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