姜黄素抑制口腔鳞状细胞癌（CAL-27）细胞侵袭与迁移的机制及其潜在临床价值-国际临床研究杂志-CSCIED科技核心评价数据库-手机版

姜黄素抑制口腔鳞状细胞癌（CAL-27）细胞侵袭与迁移的机制及其潜在临床价值

Curcumin inhibits the proliferation, migration and invasion of Cal-27 tumor cells by down regulating PD-L1 and inducing autophagy

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DOI	10.12208/j.ijcr.20250597
刊名	国际临床研究杂志 International Journal of Clinical Research
年，卷(期)	2025, 9(12)
作者	邓轩, 李浩,
作者单位	南华大学附属第二医院湖南衡阳
摘要	目的姜黄素对肿瘤细胞增殖和迁移的抑制作用已引起广泛关注;然而，其具体作用靶点和潜在机制尚不清楚。方法本研究采用Western blot分析和细胞免疫荧光染色评估蛋白表达。通过CCK-8实验、伤口愈合实验和transwell实验评估细胞增殖、迁移和侵袭能力。此外，Western blot分析用于检测与细胞增殖和迁移相关的蛋白质的变化。结果姜黄素可以抑制CAL-27细胞的增殖和迁移，同时增加其自噬水平。此外，姜黄素降低了这些细胞中PD-L1的蛋白表达。使用siRNA靶向PD-L1（siPD-L1）也上调了自噬相关蛋白的表达。值得注意的是，姜黄素和siPD-L1联合治疗比单独治疗有更明显的效果。结论本研究揭示姜黄素可能通过降低PD-L1表达水平抑制CAL-27细胞的增殖和迁移，从而促进细胞自噬。
Abstract	Objective the inhibitory effect of curcumin on the proliferation and migration of tumor cells has attracted extensive attention; However, its specific targets and potential mechanisms are still unclear. Methods Western blot analysis and cellular immunofluorescence staining were used to evaluate the protein expression. Cell proliferation, migration and invasion were evaluated by CCK-8 assay, wound healing assay and Transwell assay. In addition, Western blot analysis was used to detect the changes of proteins related to cell proliferation and migration. Results Curcumin could inhibit the proliferation and migration of Cal-27 cells, and increase the level of autophagy. In addition, curcumin reduced the protein expression of PD-L1 in these cells. SiRNA targeting PD-L1 (sipd-l1) also upregulated the expression of autophagy related proteins. It is worth noting that curcumin combined with sipd-l1 has more obvious effect than single treatment. Conclusion curcumin may inhibit the proliferation and migration of Cal-27 cells by reducing the expression of PD-L1, thereby promoting autophagy.
关键词	姜黄素；肿瘤细胞；自噬
KeyWord	Curcumin; Tumor cells; Autophagy
基金项目
页码	152-160

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引用本文

邓轩, 李浩. 姜黄素抑制口腔鳞状细胞癌（CAL-27）细胞侵袭与迁移的机制及其潜在临床价值 [J]. 国际临床研究杂志. 2025; 9; (12). 152 - 160.

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