| Abstract |
[Abstract] Objective To investigate the urinary AD-related neurofilament protein (AD7c-NTP) on different types of cognitive disorders and its relationship with the degree of hippocampus atrophy in head MRI and MRS N - acetyl - aspartate (NAA) peak level, and to explore the differential diagnosis of the three different detection in different types of cognitive impairment. Methods From July 2015 to February 2017, Alzheimers disease (AD), vascular dementia (VaD) and mild cognitive impairment (MCI) (each 30 cases) in the Eighth Hospital Affiliated to Sun Yat - sen University were divided into AD group, VaD group and MCI group, another 30 healthy subjects were selected as control group. Took urine samples from all the subjects in the morning and AD7c-NTP levels were measured by enzyme-linked immunosorbent assay, besides, head skull MRI and MRS examination were performed as well. AD7c-NTP, hippocampal atrophy MTA score, NAA peak level were statistically compared in the four groups, and correlation of urinary AD7c-NTP level with hippocampus atrophy MTA score and NAA peak level was analyzed. Results (1)The urinary AD7c-NTP level: Urinary AD7c-NTP level in MCI group was higher than that in control group, urine AD7c-NTP level in VaD group was higher than that in MCI group, and AD7c-NTP level in AD group was higher than that in VaD group, the difference was statistically significant (P<0.05); (2) Hippocampus atrophy MTA score : There was no significant difference in the MTA score between the MCI group and the control group (P>0.05), the VaD group was higher than that in MCI group, the AD group was higher than that in VaD group and the difference was statistically significant (P<0.05); (3) NAA peak level: There was no significant difference in NAA peak level between MCI group and control group (P>0.05), the VaD group was lower than that in MCI group, while the AD group was lower than that in VaD group (P<0.05); (4) The correlation of urinary AD7c-NTP levels with hippocampus atrophy MTA score and peak NAA level: there was a positive correlation between AD7c-NTP level and hippocampal atrophy MTA score (r1 = 0.336, r2 = 0.329, P<0.05); and it was negatively correlated with NAA peak level. Conclusion Urinary AD7c-NTP level in AD, VaD and MCI group is higher, and there are significant differences in urinary AD7c-NTP level, hippocampal atrophy MTA score and NAA peak level in different types of cognitive disorders, urine AD7c-NTP level changes were positive correlate with MRI, and it was negatively correlated with MRS, which can be used for the differential diagnosis of cognitive disorders.
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