International Medical Research Frontier

Objective To establish a high-performance liquid chromatography (HPLC) method for the simultaneous determination of 19 preservatives in liquid facial masks. Methods The liquid facial mask samples were extracted with methanol and separated using an XBridge C18 column. The mobile phase consisted of acetonitrile-0.2% phosphoric acid solution for elution. The contents of 19 preservatives were determined at three wavelengths of 230 nm, 254 nm, and 280 nm using a high-performance liquid chromatograph. Results The results showed that within the concentration range of 0.11~3120 μg·mL-1, all 19 preservatives exhibited good linearity with correlation coefficients exceeding 0.9990. The limits of detection were between 0.37 and 2.46 μg·g-1, and the limits of quantification were between 1.10 and 7.39 μg·g-1. The average recovery rates ranged from 96.7%~104.2%, with relative standard deviations (RSD) not exceeding 4.8%. The intra-day precision RSD ranged from 0.5%~2.8%. Conclusion The established method is sensitive, accurate, rapid, and stable, capable of simultaneously determining the contents of 19 preservatives in liquid facial masks.

Liquid Facial Mask; Preservatives; High-Performance Liquid Chromatography; Simultaneous Determination