| Abstract |
Objective To investigate the effects of Wendan Decoction on sleep and neurotransmitter levels in the hippocampal region of p-chlorophenylalanine (PCPA)-induced insomnia rats. Methods Ten of 40 SD rats were randomly selected as the normal group, and the other 30 rats were injected intraperitoneally with PCPA suspension to prepare the insomnia model, and then randomly divided into the model control group, the eszopiclone group, and the Wendan Decoction group, with 10 rats in each group. Each group was given the corresponding concentration of the drug by gavage for 7 consecutive days, and the normal group and the model control group were given the same amount of saline by gavage for 7 consecutive days. On the 7th day of drug administration, the rats were hypnotised with sodium pentobarbital after 30 min of gavage, and the sleep latency and sleep time of each group were observed. After sleep induction, the rats in each group were fasted for 12 hours, anaesthetized by intraperitoneal injection of sodium pentobarbital, and their hippocampal tissues were separated for the detection of 5-HT, GLU, and GABA indexes. Results The general state of the rats in the Wendan Decoction group was better than that of the model control group, and the change of body mass increased compared with that of the model control group (P<0.05); compared with the model control group, the sleep latency of the rats in the Wendan Decoction group was significantly shortened (P<0.05), and the sleeping time was significantly prolonged (P<0.05), and the levels of 5-hydroxytryptamine (5-HT) and γ-aminobutyric acid (GABA) in the hippocampus of the Wendan Decoction group decreased (P<0.05), and the level of glutamic acid (Glu) was significantly increased (P<0.05). decreased (P<0.05), and the level of glutamate (Glu) was significantly increased (P<0.05). Compared with the model control group, the insomnia symptoms of rats in each drug group were improved, and the above indexes were not as good as those of the normal group, but all of them had a tendency to converge to the normal group (P<0.05). Conclusion Wendan Decoction has the effect of improving sleep disorder in PCPA-induced insomnia rats.
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