International Research in Chinese Medicine

Objective The protective effect of Wumen Shenyan Oral Liquid Ⅰ on adriamycin-induced podocyte injury in rats was studied based on the miRNA-30b-3p-mediated Wnt signaling pathway. Methods The rat model of adriamycin-induced renal podocyte injury was established. Biochemical method, electron microscopy and Western blotting were used to investigate the effect of Wumen Shenyan Oral Liquid I on miRNA-30b-3p regulating Wnt/β-catenin signaling pathway and protecting podocytes by group intervention combined with miRNA mimic transfection technology. Results Compared with the model group, Wumen Nephritis Oral Liquid I significantly reduced 24 h proteinuria (P< 0.01), improved podocyte injury, and reduced renal pathological damage. The protein expression of Nephrin and Podocin in renal podocytes induced by adriamycin was inhibited (P < 0.01). In addition, compared with the model group, the expression levels of Wnt1 and β-catenin in the kidneys of rats in the miR-30b-3p overexpression plasmid injection group and the nephritis oral liquid I + miR-30b-3p overexpression plasmid injection group were significantly down-regulated (P< 0.05 or P< 0.01). Conclusion Wumen Shenyan Oral Liquid I may improve adriamycin-induced podocyte dysfunction in rats by up-regulating the expression of miR-30b-3p and inhibiting Wnt /β-catenin signaling pathway.

Wumen Shenyan Oral Liquid Ⅰ; podocyte injury; Wnt/β-catenin signaling; miR-30b-3p