基于Lin28a/let-7a轴探讨芍药软肝方抑制HepG2增值作用的研究-国际中医药研究-CSCIED科技核心评价数据库-手机版

基于Lin28a/let-7a轴探讨芍药软肝方抑制HepG2增值作用的研究

Study on the inhibitory effect of Shaoyao Ruangan prescription on HepG2 proliferation based on Lin28a/let-7a axis

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DOI	10.12208/j.ircm.20240044
刊名	国际中医药研究 International Research in Chinese Medicine
年，卷(期)	2024, 4(3)
作者	王鑫昱*,马永力,刘立权,文雯,
作者单位	浙江中医药大学附属杭州市中医院浙江杭州 ;
摘要	研究芍药软肝方对肝癌细胞HepG2增值抑制的影响及初步作用机制。方法 MTT法检测不同浓度芍药软肝方对HepG2细胞增殖的影响；qRT-PCR和Western blot检测芍药软肝方对Lin28a/let-7a轴的影响。结果芍药软肝方能够抑制HepG2细胞增殖，并诱导其凋亡；芍药软肝方能够上调let-7a表达，下调Lin28a表达。结论芍药软肝方可能通过调控Lin28a/let-7a轴抑制HepG2增值，诱导细胞凋亡，为肝癌治疗提供新的思路。
Abstract	Objective To study the effect of Shaoyao Ruangan prescription on hepatocellular carcinoma cell HepG2 proliferation inhibition and its preliminary mechanism. Methods MTT assay was used to detect the effect of Paeonia Ruangan Decoction on the proliferation of HepG2 cells. The effect of Shaoyao Ruangan prescription on Lin28a/let-7a axis was determined by qRT-PCR and Western blot. Results Shaoyao Ruangan Formula could inhibit proliferation and induce apoptosis of HepG2 cells. Shaoyao Ruangan formula could up-regulate the expression of let-7a and down-regulate the expression of Lin28a. Conclusion Shaoyao Ruangan formula may inhibit HepG2 proliferation and induce apoptosis by regulating Lin28a/let-7a axis, providing a new idea for the treatment of liver cancer.
关键词	芍药软肝方；肝癌；细胞凋亡；Lin28a；let-7a
KeyWord	Shaoyao Ruangan formula; Liver cancer; Cell apoptosis; Lin28a; let-7a
基金项目
页码	10-13

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引用本文

王鑫昱*,马永力,刘立权,文雯. 基于Lin28a/let-7a轴探讨芍药软肝方抑制HepG2增值作用的研究 [J]. 国际中医药研究. 2024; 4; (3). 10 - 13.

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