International Journal of Clinical Research

Objetive To analyze and explore the impact of insufficient HE staining and dewaxing on clinical and pathological diagnosis in routine paraffin sections. Methods: Randomly select 2 paraffin tissue samples from our department, and cut 2 white slices each. Take 1 white slice from each case as the control group and the experimental group, respectively. The slices of the control group were fully dewaxed and then prepared according to the conventional manual HE staining process. The slices of the experimental group were quickly placed in xylene and immediately removed, and entered the subsequent staining process together with the control group. After the preparation was completed, the staining effects of the two groups of slices were observed under the microscope and analyzed. Results: In the control group, sections were evenly stained with clear tissue and cell structures, which was helpful for pathological diagnosis. In the experimental group, sections were unevenly stained, and some tissues and cells were blurred or unable to show color, which seriously affected pathological diagnosis. Conclusion: As the first step of HE staining, dewaxing is the prerequisite for dyeing a paraffin section. Whether the paraffin section is thoroughly dewaxed determines the dyeing effect. If the section is not dewaxed cleanly, it is difficult for dyes and reagents to enter the tissue cells, resulting in poor dyeing effect, which is detrimental to clinical and pathological diagnosis, and is prone to misdiagnosis or missed diagnosis.

HE staining; Dewaxing; pathologic diagnosis