LAMP-2在结节性多动脉炎中的表达水平及临床意义-国际临床研究杂志-CSCIED科技核心评价数据库-手机版

LAMP-2在结节性多动脉炎中的表达水平及临床意义

The levels and clinical significance of serum LAMP- 2 in patients with polyarteritis nodosa

ES评分 0 浏览量：615 下载量：0

DOI	10.12208/j.ijcr.20230201
刊名	国际临床研究杂志 International Journal of Clinical Research
年，卷(期)	2023, 7(5)
作者	朱晴,洪静,朱彬,吴婷,刘莎莎,李南方*,
作者单位	新疆维吾尔自治区人民医院高血压中心新疆乌鲁木齐 ; 新疆高血压研究所新疆乌鲁木齐 ; 国家卫生健康委高血压诊疗研究重点实验室新疆乌鲁木齐 ; 新疆维吾尔自治区重点实验室“新疆高血压病研究实验室” 新疆乌鲁木齐 ; 新疆高血压（心脑血管）疾病临床医学研究中心新疆乌鲁木齐 ;
摘要	探讨血清溶酶体相关膜蛋白2（LAMP-2）在结节性多动脉炎（PAN）患者中的表达及其临床意义。方法本研究共纳入26例PAN患者作为病例组。同期收集与之性别、年龄相匹配的健康体检者26例作为对照（HC）组。根据伯明翰血管炎活动期评分（BVAS）量表对PAN的疾病活动程度进行评估。采用ELISA检测所有研究对象血清中LAMP-2水平。结果 PAN患者血清LAMP-2水平显著高于HC，差异有显著统计学意义 [0.66 (0.49-2.95) vs 0.17 (0.10-0.32)，Z=4.813，P<0.001]。相关性分析表明，血清LAMP-2水平与BAVS（r=0.438，P=0.025）、Hs-CRP（r=0.592，P=0.001）和ESR（r=0.406，P=0.039）呈显著正相关，而与Scr（r=0.198，P=0.332）以及24小时蛋白尿（r=0.225，P=0.302）无相关性。结论与对照组相比，PAN患者的血清LAMP-2水平升高，且与疾病活动指标呈正相关。LAMP-2可能是一种新型的生物标记物用于PAN患者的诊断及疾病活动评估。
Abstract	Objective: To investigate the levels and clinical significance of serum lysosome-associated membrane protein-2 (LAMP-2) in patients with polyarteritis nodosa (PAN). Methods A total of 26 PAN patients were enrolled as case group. At the same time, 26 healthy persons matched with sex and age were collected as health controls (HC) group.The disease activity of PAN was assessed by the birmingham vasculitis activityscore (BVAS). The level of LAMP-2 in serum of all subjects was detected by ELISA. Results The level of serum LAMP-2 in PAN patients was significantly higher than in HC, the difference was statistically significant [0.66 (0.49-2.95) vs 0.17 (0.10-0.32), Z = 4.813, P < 0.001]. The correlation analysis showed that serum LAMP-2 levels were positively correlated with BAVS (r=0.438, P=0.025), Hs-CRP (r=0.592, P=0.001) and ESR (r=0.406, P=0.039), but not with Scr (r=0.198, P=0.332) and 24-hour proteinuria (r=0.225, P=0.302). Conclusion Compared with the control group, the serum LAMP-2 level of PAN patients was increased, which was positively correlated with disease activity indicators. LAMP-2 May be a novel biomarker for diagnosis and disease activity assessment of PAN patients.
关键词	溶酶体相关膜蛋白2；结节性多动脉炎；疾病活动
KeyWord	Lysosome-associated membrane protein 2; Polyarteritis nodosa; Disease activity
基金项目
页码	1-5

参考文献
相关文献

[1] Karadag O, Jayne DJ. Polyarteritis nodosa revisited: a review of historical approaches, subphenotypes and a research agenda
[J]. Clinical and experimental rheumatology, 2018, 36 Suppl 111(2):135-142.

[2] 朱彬. 系统性血管炎患者临床特点及生物标记物的相关研究
[D].新疆医科大学，2019.

[3] Zhu Qing; Liu Shasha; Heizhati Mulalibieke; Yao Xiaoguang; Wang Menghui; Luo Qin; Wang Lei; Zhang Delian; Chang Guijuan; Li Nanfang; Systemic Vasculitis: An Important and Underestimated Cause of Malignant Hypertension
[J]. Cardiovascular Innovations and Applications (CVIA), 2019, 4(2):99-108.

[4] Benarous L, Terrier B, Laborde-Casterot H, et al. Employment, work disability and quality of life in patients with ANCA-associated vasculitides. The EXPOVAS study
[J]. Clinical and experimental rheumatologyl, 2017, 35 Suppl 103(1):S40-46.

[5] Barra LJ, Bateman EA, Rohekar S, et al. Assessment of work limitations and disability in systemic vasculitis
[J]. Clinical and experimental rheumatologyl, 2016,34(3 Suppl 97):S111-4.

[6] 刘莎莎. MCP-1与系统性血管炎的相关性研究
[D].新疆医科大学,2019.

[7] 桂雅迪, 帅宗文. 溶酶体相关膜蛋白-2及其自身抗体的研究进展
[J].中华风湿病学杂志, 2016, 20(6):424-428.

[8] Gonzalez A, Valeiras M, Sidransky E, et al. Lysosomal Integral Membrane Protein-2: A New Player in Lysosome- Related Pathology
[J].Molecular genetics and metabolism, 2014,111(2):84-91.

[9] Gibson Kristen M,Kain Renate,Luqmani Raashid A,et al. Autoantibodies Against Lysosome Associated Membrane Protein-2 (LAMP-2) in Pediatric Chronic Primary Systemic Vasculitis.Frontiers in immunology.2020;11：624758.doi:10.3389/fimmu.2020.624758

[10] Kain R, Matsui K, Exner M, et al. A novel class of autoantigens of anti-neutrophil cytoplasmic antibodies in necrotizing and crescentic glomerulonephritis: the lysosomal membrane glycoprotein h-lamp-2 in neutrophil granulocytes and a related membrane protein in glomerular endothelial cells
[J]. Journal of experimental medicine, 1995,181(2):585-597.

[11] Roth AJ, Brown MC, Smith RN, et al. Anti–LAMP-2 Antibodies Are Not Prevalent in Patients With Antineutrophil Cytoplasmic Autoantibody Glomerulone- phritis
[J]. Journal of the American society of nephrology, 2012, 23(3):545-555.

[12] Wang Lu,Wang Jingbo,Shi Yongquan,et al.Identification of a primary biliary cirrhosis associated protein as lysosome- associated membrane protein-2.Journal of Proteomics.2013;91：569-79.

doi:10.1016/j.jprot.2013.08.019

[13] Lee Jong-Jer,Ishihara Kenji,Notomi Shoji,et al. Lysosome- associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 2020;521(2):414-419.doi:10.1016/j.bbrc. 2019. 10.138.

[14] Lightfoot RW Jr, Michel BA, Bloch DA, et al. The American College of Rheumatology 1990 criteria for the classification of polyarteritis nodosa
[J]. Arthritis Rheumatology, 1990, 33(8):1088-1093.

[15] Jennette JC, Falk RJ, Bacon PA, et al. 2012 revised International Chapel Hill Consensus Conference Nomenclature of Vasculitides
[J]. Arthritis Rheumatology, 2013, 65(1):1-11.

[16] Mukhtyar C, Lee R, Brown D, et al. Modification and validation of the Birmingham Vasculitis Activity Score (version 3)
[J]. Annals of the rheumatic diseases, 2009, 68(12):1827-1832.

[17] Monach PA. Biomarkers in vasculitis
[J]. Current Opinion in Rheumatology, 2014,26(1):24.

[18] 唐莎, 张莹, 施维维. 溶酶体相关膜蛋白-2抗体诱导中性粒细胞胞外捕网形成的实验研究
[J].免疫学杂志,2012,(11):968-971.

[19] Kawakami T, Takeuchi S, Arimura Y, et al. Elevated antilysosomal-associated membrane protein-2 antibody levels in patients with adult Henoch-Schönlein purpura
[J]. British journal of dermatology, 2012, 166(6):1206-1212.

[20] 陈熙, 胡志东. 溶酶体相关膜蛋白2抗体在ANCA相关性肾炎患者血清中的表达水平及意义
[J].中华肾脏病杂志,2014,30(7):494-498.

[21] 牛蒙, 李娜, 孙建平, 等. 溶酶体相关膜蛋白2抗体在抗中性粒细胞胞质抗体相关性小血管炎患者血清中的表达及临床意义
[J].中华风湿病学杂志, 2016, 20(5): 321-324.

[22] Peschel A, Basu N, Benharkou A, et al. Autoantibodies to hLAMP-2 in ANCA-Negative Pauci-Immune Focal Necrotizing GN
[J].Journal of the American society of nephrology, 2014,25(3):455-463.

[23] Kain R, Tadema H, Mckinney EF, et al. High prevalence of autoantibodies to hLAMP-2 in anti-neutrophil cytoplasmic antibody-associated vasculitis
[J]. Journal of the American society of nephrology, 2012,23(3):556-566.

[24] Kain R, Exner M, Brandes R, et al. Molecular mimicry in pauci-immune focal necrotizing glomerulonephritis
[J]. Nature Medicine, 2008,14(10):1088-1096.

引用本文

朱晴,洪静,朱彬,吴婷,刘莎莎,李南方*. LAMP-2在结节性多动脉炎中的表达水平及临床意义 [J]. 国际临床研究杂志. 2023; 7; (5). 1 - 5.

文献评论

相关学者

相关机构