International Journal of Clinical Research

To explore the physiological mechanism of Ginseng Sini Decoction decoction decoction treating sub-health. Methods: Based on TCM(Traditional Chinese Medicine) Network Pharmacological Analysis platform (TCMSP), we searched the chemical constituents and target sites of four Chinese herbs (ginseng, aconite, ginger and licorice) in GSSD. The complex target network and protein interaction (PPI) network were constructed, gene ontology (GO) functional enrichment analysis was performed, Kyoto gene and genome (KEGG) pathway enrichment analysis was performed, and the molecular mechanism of protein energy transfer and gene vector were analyzed. Results: The complex target network contained 137 compounds and 60 corresponding targets, the key targets involved prostaglandin G/H synthase 2(PTGS2), estrogen receptor, calmodulin and so on. The PPI network contains 39 proteins, the key proteins include Jun, Ar, NCOA1 protein and so on. GO functional enrichment analysis produced 167 acting entities. KEGG pathway enrichment screened 70 signaling pathways involved in calcium signaling pathways, neuroactive ligand receptor interactions, cancer pathways, spectral analysis combined with DNA energy relaxation mechanisms, etc. The results of this study preliminarily verified the molecular and biophysical mechanism of GSSD in the prevention and treatment of sub-health, and provided reference information and basis for further exploration of its mechanism.

Ginseng Sini Decoction (GSSD); Network pharmacology; Protein interaction Network (PPI); Gene ontology (GO); Signal pathway; Molecular dynamics; DNA energy relaxation