The expression of miR-21 in brain glioma cells and its effect of PI3K/AKT signal pathway-Journal of Psychiatry and Brain Science-CSCIED科技核心评价数据库-手机版

The expression of miR-21 in brain glioma cells and its effect of PI3K/AKT signal pathway

The expression of miR-21 in brain glioma cells and its effect of PI3K/AKT signal pathway

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DOI	10.20900/jpbs.20160005
刊名	Journal of Psychiatry and Brain Science JPBS
年，卷(期)	2016, 1(1)
作者	Feng Liu,Shimin Zhang*,Junjie Luo,
作者单位	1 Department of Neurosurgery, Xiangya 3rd Hospital Changsha, Hunan 410013,China; 2 Division of Molecular Pathology, Walter Reed National Military Medical Center, Bethesda, MD 20889; 3 Department of Neurosurgery, people's Hospital Affiliated to Hubei Medical University,442000,China
Abstract	Background: Brain glioma is a common primary intracranial tumor with unfavorable prognosis. MicroRNA-21 (miR-21) is a small RNA molecule involved in post-transcriptional modulation and is closely associated with cell growth and differentiation. Recent studies have shown that miR-21 expression affects tumor proliferation and growth via the PI3K/AKT signaling pathway. This study investigated the expression of miR-21 in glioma and its effects on the PI3K/AKT pathway and cell proliferation. Methods: A total of 35 brain glioma patients were recruited from March 2013 to November 2014 at our hospital. In situ hybridization (ISH) and RT-PCR were used to detect the expression of miR-21 in both tumors and adjacent brain tissues. Human brain U251 glioma cells were transfected with anti-miR-21 to suppress miR-21 expression, followed by immunofluorescent assay of PI3K and AKT expression profiles. Flow cytometry was employed to detect the change between different stages of the cell cycle. Results: ISH results showed significantly elevated miR-21 expression level in glioma tissues compared to normal brain tissues. RT-PCR analysis revealed a 3.5-fold increase in miR-21 in the anti-miR-21 transfected glioma cells compared to untransfected cells. After silencing miR-21 in U251 cells, immunofluorescent assay indicated that the expression of PI3K and AKT significantly decreased. Flow cytometry revealed an increased number of cells at G0/G1 phase in anti-miR-21 transfected U251 cells, suggesting suppressed cell division ability. Conclusion: MiR-21 expression was found to be increased in brain glioma tissues, accompanied with over-activation of PI3K/AKT signaling and proliferation of glioma cells.
KeyWord	Brain glioma; MicroRNA-21; PI3K/AKT signal pathway; Cell proliferation
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引用本文

Feng Liu,Shimin Zhang*,Junjie Luo. The expression of miR-21 in brain glioma cells and its effect of PI3K/AKT signal pathway, Journal of Psychiatry and Brain Science. 2016; 1; (1). https://doi.org/10.20900/jpbs.20160005.

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