CBGG

The aim of this research was the identification of DNA markers to Antocyanin 1 (Ant1) tomato orthologs in 30 Capsicum annuum, 30 Solanum melongena and 1 Solanum aethiopicum accessions with different anthocyanin accumulation. A search for orthologs to the Ant1 allele in the GenBank database revealed the following closest nucleotide sequences of Myb113-like TF: in C. annuum, mRNA XM_016689227, mRNA NM_001324618; in S. melongena, mRNA KT259043. In Capsicum spp. samples, 4 SNP and a single base deletion in exon 3 of the Myb113-like 1 allele and 2 SNP in exon 4 of the Myb113-like2 allele were detected in forms with the impaired anthocyanin synthesis, based on their phenotype.In S. melongena samples, a 6 bp deletion at the end of exon 1 of the Myb-1 gene was detected in accessions with a green fruit coloration. A 26 bp deletion between intron 1 and exon 2, 11 SNP in exon regions and a 52 bp insertion at the beginning of intron 2 were detected in forms with a white fruit coloration. A 9-nucleotide insertion and 11 SNP in exon 3 of the Myb-1 gene were detected in the S. aethiopicum accession with no anthocyanin accumulation in fruits.Optimal SCAR and CAPS markers were developed to identify a number of genetic polymorphisms of Myb-like TF genes that might cause the impaired anthocyanin synthesis in pepper and eggplant. CAPS Myb 113-AccI and dCAPS Myb 113-AciI markers allow to identify C.annuum accessions without an anthocyanin coloration of fruits. SCAR MybMel and CAPS Mybmel-PstI markers allow to identify S. melongena accessions with white and green fruit colorations.Samples with different allelic polymorphism combinations were selected as models for the further study of the genetic regulation of anthocyanin accumulation in various parts of plants and for improved plant breeding.

Solanum lycopersicum; Capsicum annuum; Solanum melongena; regulation of anthocyanin synthesis; DNA markers