International Journal of Medicine and Data

Objective To explore the effect of PCR detection of rubella virus and judge its application value. Methods according to the inclusion and exclusion criteria of the study, 488 rubella patients admitted to our hospital from January 2007 to June 2022 were selected as the study subjects. All patients had complete medical records, numbered 1-488. All patients underwent PCR and ELISA tests at the same time. The PCR test results were regarded as the data of the study group, and the ELISA test data were regarded as the data of the control group. The coincidence rate, specificity and sensitivity of the two groups of data were compared. Results the cell culture method was used as the gold standard in the study. 488 patients were rubella virus positive. In the clinical analysis combined with case data, the coincidence rate of PCR examination was higher than that of ELISA antibody detection. At the same time, the sensitivity of PCR examination method was significantly higher, and the difference was statistically significant (P < 0.05). Since there were 0 true negative cases, the two examination methods did not involve calculation. Conclusion the application of fluorescence quantitative PCR is convenient, with high accuracy, sensitivity and specificity, and accurate quantification. It can effectively detect rubella virus infection and provide effective reference information for clinical treatment and infection prevention. The clinical effect of PCR detection is good, and it has the value of popularization and application.

rubella virus; Nucleic acids; PCR detection; Check compliance rate; Sensitivity; Specificity