Journal of Modern Nursing MedicineJPCNS

Objective Mycoplasma pneumoniae is a major pathogen caused by infection of the lower respiratory tract. When patients suffer from mycoplasma pneumoniae disease, it will cause local infection. In severe cases, it will lead to the damage of the patients organs and systems. This article explores the clinical application value of PCR combined with enzyme-linked immunosorbent assay in Mycoplasma pneumoniae infection patients. Methods The samples selected from January 2020 - May 2022 in our hospital (north sichuan medical college affiliated hospital clinical laboratory) for inspection and treatment of 407 cases of children with respiratory tract infection, for all the subjects to different detection methods, implementation of PCR method respectively and enzyme-linked immunosorbent assay for testing, comparing PCR method, enzyme-linked immunosorbent assay index test results. Results The positive coincidence rate of PCR was 86.36%, and the positive coincidence rate of enzyme-linked immunosorbent assay was 88.19%, P>0.05 indicating that there was no comparative significance. The results of PCR and MP-igm for Mycoplasma pneumoniae detection were statistically significant (P<0.05). The results of PCR and enzyme-linked immunosorbent assay for Mycoplasma pneumoniae detection were in poor agreement. Conclusion the children with mycoplasma pneumoniae infection in PCR method combined with enzyme-linked immunosorbent assay to detect high value for clinical inspection, can significantly improve diagnostic accuracy, doctor for early diagnosis and differential provided valuable reference, to control the condition, improve prognosis has promoting effect, it is of great significance in clinical diagnosis and treatment.

Mycoplasma pneumoniae; The PCR method; Enzyme-linked immunosorbent assay; Test value