益肺扶正解毒法防治肺癌化疗骨髓抑制的临床观察及对Wnt/β-catenin 通路的机制影响-国际中医药研究-CSCIED科技核心评价数据库-手机版

益肺扶正解毒法防治肺癌化疗骨髓抑制的临床观察及对Wnt/β-catenin 通路的机制影响

Clinical observation of Yifei Fuzheng Jiedu method for preventing and treating chemotherapy-induced myelosuppression in lung cancer patients and its mechanistic impact on the Wnt/β-catenin pathway

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DOI	10.12208/j.ircm.20260036
刊名	国际中医药研究 International Research in Chinese Medicine
年，卷(期)	2026, 6(2)
作者	程玉峰, 郜曦, 郝庆伟, 何蕊, 怀利, 李艳彬, 夏奔, 夏友宏,
作者单位	亳州市中医院呼吸科安徽亳州;
摘要	目的本研究拟探讨益肺扶正解毒法对肺癌化疗相关骨髓抑制的干预效果，并重点研究其对WNT/β-catenin信号通路的影响。方法采用随机对照试验设计，将60例接受化疗的肺癌患者随机分为两组，对照组30例仅接受常规支持治疗，治疗组30例在常规治疗基础上联合益肺扶正饮（治法为益肺扶正解毒法）。观察两组外周血象（白细胞、血小板、血红蛋白计数）变化，并在治疗期间动态观察两组治疗前后中医证候积分、功能状态（KPS）评分；借助采用流式细胞术动态检测外周血CD34⁺造血干细胞比例，同时通过荧光定量PCR动态检测血清中Wnt3a、c-Myc的mRNA表达水平。结果与对照组相比，治疗组患者化疗后外周血象指标下降幅度更小（P<0.05），中医证候积分明显下降，KPS评分明显著提升（P<0.05）。机制分析显示，与对照组相比，治疗组干预14天后，Wnt3a、c-Myc mRNA表达及外周血CD34⁺细胞比例均接近正常水平，差异无统计学意义（P>0.05）。组内比较显示：治疗后7天，两组各指标均较治疗前明显改变（P<0.05）；治疗后14天，对照组各指标仍显著异于治疗前及治疗后7天（P<0.05），而治疗组各指标仅与治疗后7天存在显著差异（P<0.05），且两组各时间点比较差异均有统计学意义（P<0.05）。结论益肺扶正解毒法能够缓解肺癌患者化疗后骨髓抑制程度，提高机体免疫力，改善生活质量，其作用机制可能与上调 Wnt3a、c-Myc 表达，提升外周血CD34+细胞比例有关。
Abstract	Objective This prospective randomized controlled trial was designed to explore the clinical efficacy and underlying mechanism of Yifei Fuzheng Jiedu Therapy in mitigating chemotherapy-induced myelosuppression in patients with lung cancer, focusing on its regulatory effects on key molecules within the Wnt/β-catenin signaling pathway. Methods A randomized controlled trial was designed. Sixty chemotherapy-treated lung cancer patients were randomized into control (n=30) and treatment (n=30) groups. The control group received conventional supportive care only, while the treatment group was supplemented with Yifei Fuzheng Yin (therapeutic principle: YFJ therapy). Peripheral blood cell counts were evaluated pre- and post-treatment, and TCM syndrome scores and KPS scores were dynamically tracked. CD34+ hematopoietic stem cells were dynamically analyzed by flow cytometry, and Wnt3a/c-Myc mRNA expression was dynamically measured by qRT-PCR. Results Compared with the control group, patients in the treatment group had a smaller decrease in peripheral blood indicators after chemotherapy (P<0.05), a significant reduction in TCM syndrome scores, and a marked improvement in KPS scores (P<0.05).Following 14 days of intervention, Wnt3a/c-Myc mRNA expression and CD34+ cell proportion in the treatment group returned to normal levels, with no inter-group difference (P>0.05).Intra-group analysis demonstrated significant changes in all indicators at 7 days post-treatment vs baseline in both groups (P<0.05). At 14 days, control group indicators still differed from baseline and 7-day values (P<0.05), whereas treatment group indicators differed in 7-day values (P<0.05). All inter-group comparisons at each time point were significant differences(P<0.05). Conclusion Our findings indicate that YFJ herapy mitigates chemotherapy-related myelosuppression, boosts immune function, and improves quality of life in lung cancer patients. Its mechanism could involve upregulating Wnt3a/c-Myc expression and increasing peripheral blood CD34+ cell proportion.
关键词	肺癌；临床观察；WNT/β-catenin通路；骨髓抑制；益肺扶正解毒法
KeyWord	Lung cancer; Clinical observation; WNT/β-catenin pathway; Bone marrow suppression; Yifei Fuzheng Jiedu Therapy
基金项目
页码	66-74

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程玉峰, 郜曦, 郝庆伟, 何蕊, 怀利, 李艳彬, 夏奔, 夏友宏. 益肺扶正解毒法防治肺癌化疗骨髓抑制的临床观察及对Wnt/β-catenin 通路的机制影响 [J]. 国际中医药研究. 2026; 6; (2). 66 - 74.

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