血浆长链非编码RNA AFAP1-AS1对鼻咽癌的诊断价值-国际临床研究杂志-CSCIED科技核心评价数据库-手机版

血浆长链非编码RNA AFAP1-AS1对鼻咽癌的诊断价值

Plasma long non-coding RNA AFAP1-AS1 as a potential diagnostic biomarker for nasopharyngeal carcinoma

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DOI	10.12208/j.ijcr.20260015
刊名	国际临床研究杂志 International Journal of Clinical Research
年，卷(期)	2026, 10(1)
作者	陈锡山, 江舟, 林欢, 陆颖, 许卓华,
作者单位	广西医科大学第四附属医院肿瘤科广西柳州
摘要	目的探讨血浆长链非编码RNA AFAP1-AS1在鼻咽癌（NPC）中的诊断价值及其与临床病理特征的关系。方法采用病例对照研究设计，纳入2019年1月至2019年10月本院初治鼻咽癌患者39例及年龄、性别匹配的健康对照20例，排除吸烟、EBV感染及其他恶性肿瘤病史。采用实时荧光定量 PCR 检测血浆 AFAP1-AS1 表达，分析其与临床病理特征的关联性，并通过受试者工作特征（ROC）曲线评估诊断效能，基于 Youden 指数确定最佳临界值。结果鼻咽癌组AFAP1-AS1表达水平显著高于健康对照组（2.57±0.89 vs. 0.78±0.19，t=12.097，P<0.01）。吸烟患者AFAP1-AS1表达（3.29±0.37）显著高于非吸烟患者（2.36±0.89，t=-4.568，P<0.01），但与性别、年龄、T/N/TNM分期无关（P>0.05）。ROC曲线分析显示，AFAP1-AS1诊断NPC的AUC为0.964（95% CI: 0.911–1.000），最佳临界值为1.1389，灵敏度79.5%，特异度90.0%，阳性预测值93.9%、阴性预测值69.2%，诊断准确度83.1%。结论血浆AFAP1-AS1在鼻咽癌中高表达，且与吸烟状态显著相关，其作为非侵入性生物标志物对NPC诊断具有较高临床应用潜力。
Abstract	Objective To investigate the diagnostic value of plasma long non-coding RNA (lncRNA) AFAP1-AS1 in nasopharyngeal carcinoma (NPC) and its association with clinicopathological features. Methods From January 2019 to October 2019, 39 treatment-naive NPC patients and 20 healthy controls were enrolled. Plasma AFAP1-AS1 expression was detected by quantitative real-time PCR (qRT-PCR). Associations with clinicopathological features were analyzed, and diagnostic efficacy was evaluated using receiver operating characteristic (ROC) curves with the Youden index to determine the optimal cutoff value. Results AFAP1-AS1 expression was significantly higher in NPC patients than in controls (2.57±0.89 vs. 0.78±0.19, t=12.097, P<0.01). Smokers exhibited higher AFAP1-AS1 expression (3.29±0.37) than non-smokers (2.36±0.89, t=-4.568, P<0.01), while no associations were observed with gender, age, T/N/TNM staging (P>0.05). ROC curve analysis showed an area under the curve (AUC) of 0.964 (95% CI: 0.911–1.000), with an optimal cutoff value of 1.1389. At this cutoff, the sensitivity was 79.5%, specificity 90.0%, positive predictive value 93.9%, negative predictive value 69.2%, and diagnostic accuracy 83.1%. Conclusion Plasma AFAP1-AS1 is highly expressed in NPC and significantly associated with smoking status. As a non-invasive biomarker, it demonstrates promising clinical potential for NPC diagnosis.
关键词	鼻咽癌；AFAP1-AS1；长链非编码RNA；诊断
KeyWord	Nasopharyngeal carcinoma; AFAP1-AS1; Long non-coding RNA; Diagnosis
基金项目
页码	52-57

参考文献
相关文献

[1] Chen YP, Chan A, Le QT, Blanchard P, Sun Y, Ma J. Nasopharyngeal carcinoma. Lancet. 2019. 394(10192): 64-80.

[2] Wong K, Hui EP, Lo KW, et al. Nasopharyngeal carcinoma: an evolving paradigm. Nat Rev Clin Oncol. 2021. 18(11): 679-695.

[3] Chua M, Wee J, Hui EP, Chan A. Nasopharyngeal carcinoma. Lancet. 2016. 387(10022): 1012-1024.

[4] 李键锋. 探究鼻咽癌患者治疗前、治疗中、治疗后EBV-DNA截断值及生存预后差异 ,2024.

[5] Li W, Duan X, Chen X, et al. Immunotherapeutic approaches in EBV-associated nasopharyngeal carcinoma. Front Immunol. 2022. 13: 1079515.

[6] Wang H, Wang W, Fan S. Emerging roles of lncRNA in Nasopharyngeal Carcinoma and therapeutic opportunities. Int J Biol Sci. 2022. 18(7): 2714-2728.

[7] Fang M, Zhang M, Wang Y, et al. Long Noncoding RNA AFAP1-AS1 Is a Critical Regulator of Nasopharyngeal Carcinoma Tumorigenicity. Front Oncol. 2020. 10: 601055.

[8] Zhang JY, Weng MZ, Song FB, et al. Long noncoding RNA AFAP1-AS1 indicates a poor prognosis of hepatocellular carcinoma and promotes cell proliferation and invasion via upregulation of the RhoA/Rac2 signaling. Int J Oncol. 2016. 48(4): 1590-8.

[9] Abdul S, Majid A, Wang J, Liu Q, Sun MZ, Liu S. Bidirectional interaction of lncRNA AFAP1-AS1 and CRKL accelerates the proliferative and metastatic abilities of hepatocarcinoma cells. J Adv Res. 2020. 24: 121-130.

[10] Li Y, Zhu Z, Hou X, Sun Y. LncRNA AFAP1-AS1 Promotes the Progression of Colorectal Cancer through miR-195-5p and WISP1. J Oncol. 2021. 2021: 6242798.

[11] 王志鹏. 血浆lncRNA AFAP1-AS1和lncRNA SOX2OT水平测定诊断非小细胞肺癌 ,2021.

[12] Tasnim A, Sumaiya AA, Noman AA, et al. A Comparative Meta-Analysis on the Association of lncRNAs MALAT1, HOTAIR, and AFAP1-AS1 With the Risk of Developing Lymph Node Metastasis in Lung Cancer. Cancer Rep (Hoboken). 2024. 7(12): e70091.

[13] 江飞云, 丁叶屏, 刘星宇, 蔡文, 吴明彩. AFAP1-AS1在卵巢癌组织中的表达水平及其对卵巢癌生物学行为的影响. 解剖学研究. 2025. 47(01): 43-50.

[14] 李志明, 龙凤, 何建新, 黄勇, 孙少康. 长链非编码RNA AFAP1-AS1在癌症中作用及其机制的研究进展. 中国癌症杂志. 2021. 31(04): 350-360.

[15] 付丛, 张行炜. 长链非编码RNA AFAP1-AS1在口腔鳞状细胞癌中的表达及临床意义. 口腔医学研究. 2016. 32(09): 983-986.

[16] Zhang K, Liu P, Tang H, et al. AFAP1-AS1 Promotes Epithelial-Mesenchymal Transition and Tumorigenesis Through Wnt/β-Catenin Signaling Pathway in Triple-Negative Breast Cancer. Front Pharmacol. 2018. 9: 1248.

[17] 陈少仪, 麦如蓝, 赵淑芬, 田峰, 黄穗平. 2017-2021年佛山市顺德区鼻咽癌筛查人群EB病毒流行状况及影响因素. 社区医学杂志. 2025. 23(01): 9-15.

引用本文

陈锡山, 江舟, 林欢, 陆颖, 许卓华. 血浆长链非编码RNA AFAP1-AS1对鼻咽癌的诊断价值 [J]. 国际临床研究杂志. 2026; 10; (1). 52 - 57.

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