荞麦玉米丸纳米制剂对骨质疏松大鼠的改善作用研究-国际中医药研究-CSCIED科技核心评价数据库-手机版

荞麦玉米丸纳米制剂对骨质疏松大鼠的改善作用研究

Research on the ameliorative effect of buckwheat and corn pill nano-formulations on osteoporotic rats

ES评分 0 浏览量：61 下载量：0

DOI	10.12208/j.ircm.20250040
刊名	国际中医药研究 International Research in Chinese Medicine
年，卷(期)	2025, 5(4)
作者	彭家伟², 史小方¹, 宋存峰³, 周宁¹, 朱翔¹,
作者单位	1 北京煦堂生物科技有限公司北京 2 纳米技术及应用国家工程研究中心上海 3 上海交通大学上海
摘要	目的探讨荞麦玉米丸纳米制剂对骨质疏松模型大鼠骨密度、骨微结构及骨代谢指标的改善作用。方法采用双侧睾丸切除联合维生素A酸灌胃构建大鼠骨质疏松模型。将造模成功的大鼠随机分为模型组和给药组，并设正常对照组。给药组灌胃荞麦玉米丸纳米制剂（12 mg/kg，每日3次）干预75天。干预结束后，通过Micro-CT检测股骨骨矿物质密度（BMD）、组织骨密度（TMD）、骨小梁数量（Tb.N）、厚度（Tb.Th）、分离度（Tb.Sp）及结构模型指数（SMI）；采用ELISA法检测血清骨碱性磷酸酶（BALP）和抗酒石酸酸性磷酸酶5b（TRACP-5b）水平。结果与模型组相比，给药组大鼠的股骨BMD、TMD、Tb.N和Tb.Th均显著升高（P<0.05或P<0.01），Tb.Sp和SMI显著降低（P<0.01）。血清骨形成标志物BALP与骨吸收标志物TRACP-5b水平亦显著恢复（P<0.01），接近正常对照组水平。结论荞麦玉米丸纳米制剂能有效改善骨质疏松大鼠的骨密度与骨微结构，其作用机制可能与调节骨形成和骨吸收平衡有关，具有良好的开发应用前景。
Abstract	Objective To investigate the ameliorative effects of a Buckwheat and Corn nano-preparation on bone mineral density (BMD), bone microstructure, and bone metabolism indices in a rat model of osteoporosis. Methods An osteoporosis model was established in rats using bilateral orchidectomy combined with vitamin A acid gavage. Successfully modeled rats were randomly divided into a model group and a treatment group, with a normal control group also established. The treatment group was administered the Buckwheat and Corn nano-preparation (12 mg/kg, three times daily) via gavage for 75 days. After the intervention, femoral BMD, tissue mineral density (TMD), trabecular bone number (Tb.N), thickness (Tb.Th), separation (Tb.Sp), and structure model index (SMI) were measured using Micro-CT. Serum levels of bone alkaline phosphatase (BALP) and tartrate-resistant acid phosphatase 5b (TRACP-5b) were detected by ELISA. Results Compared with the model group, the treatment group showed significantly increased femoral BMD, TMD, Tb.N, and Tb.Th (P < 0.05 or P < 0.01), and significantly decreased Tb.Sp and SMI (P < 0.01). Serum levels of the bone formation marker BALP and the bone resorption marker TRACP-5b were also significantly restored (P < 0.01), approaching levels observed in the normal control group. Conclusion The Buckwheat and Corn nano-preparation can effectively improve bone density and microstructure in osteoporotic rats. Its mechanism of action is likely associated with regulating the balance between bone formation and bone resorption, demonstrating good potential for development and application.
关键词	中药复方纳米制剂；荞麦玉米丸；骨质疏松；动物实验
KeyWord	Traditional Chinese medicine compound nanopreparation; Buckwheat and corn pills; Osteoporosis; Animal experimentation
基金项目
页码	33-39

参考文献
相关文献

[1] Foessl I, Dimai HP, Obermayer-Pietsch B. Long-term and sequential treatment for osteoporosis
[J]. Nat Rev Endocrinol, 2023, 19(9): 520-533.

[2] 中国骨质疏松症流行病学调查及“健康骨骼”专项行动结果发布
[J]. 中华骨质疏松和骨矿盐疾病杂志, 2019, 12(04): 317-318.

[3] 中华医学会骨质疏松和骨矿盐疾病分会, 章振林. 原发性骨质疏松症诊疗指南（2022）
[J]. 中国全科医学, 2023, 26(14): 1671-1691.

[4] Song S, Guo Y, Yang Y, et al. Advances in pathogenesis and therapeutic strategies for osteoporosis
[J]. Pharmacol Ther, 2022, 237: 108168.

[5] 阎红. 荞麦的应用研究及展望
[J]. 食品工业科技, 2011, 32(01): 363-365.

[6] 韩旭, 李英华, 洪洋. 富含黄酮类化合物的中药材在骨质疏松治疗中的研究进展
[J]. 中医临床研究, 2025, 17(18): 107-114.

[7] 方晔, 邹斌, 赵劲民, 等. Α-玉米赤霉醇在雌激素缺乏所致骨质疏松症中的研究进展
[J]. 广西医科大学学报, 2014, 31(05): 870-872.

[8] Bakir M, Dawalibi A, Mufti MA, et al. Nano-drug delivery systems for bone metastases: Targeting the tumor-bone microenvironment
[J]. Pharmaceutics, 2025, 17(5).

[9] Zheng L, Zhuang Z, Li Y, et al. Bone targeting antioxidative nano-iron oxide for treating postmenopausal osteoporosis
[J]. Bioact Mater, 2022, 14: 250-261.

[10] 盛岑卓, 汪叶青, 姚晓聪, 等. 补益牛膝丸治疗骨质疏松症的研究
[J]. 中国骨质疏松杂志, 2025, 31(01): 15-20+54.

[11] 郑修竹, 朱春雨, 梁彦博, 等. 冬凌草甲素对绝经后骨质疏松小鼠肠道菌群的影响
[J]. 中国骨质疏松杂志, 2025, 31(06): 799-805.

[12] 赵阳, 杨勇, 孟志成, 等. 骨质疏松症动物模型构建方法的研究进展
[J]. 山东医药, 2023, 63(04): 95-99.

[13] Cao L, Yang K, Yuan W, 等. Melatonin mediates osteoblast proliferation through the stim1/orai1 pathway
[J]. Front Pharmacol, 2022, 13: 851663.

[14] 谢梦洲. 中医药膳学（新世纪第三版）.
[M]: 中国中医药出版社, 2016:39,40,115.

[15] Abdul-Fattah Baraka N, Fathallah Ahmed N, Ismail Hussein S. The effect of rutin hydrate on glucocorticoids induced osteoporosis in mandibular alveolar bone in albino rats (radiological, histological and histochemical study)
[J]. Saudi Dent J, 2022, 34(6): 464-472.

[16] Xiao Y, Wei R, Yuan Z, 等. Rutin suppresses fndc1 expression in bone marrow mesenchymal stem cells to inhibit postmenopausal osteoporosis
[J]. Am J Transl Res, 2019, 11(10): 6680-6690.

[17] Zong S, Wei B, Xiong C, 等. The role of alpha-zearalanol in reversing bone loss induced by ovarian hormone deficiency in rats
[J]. J Bone Miner Metab, 2012, 30(2): 136-143.

[18] 邹斌, 宗少晖, 曾高峰, 等. Α-玉米赤霉醇影响小鼠骨髓间充质干细胞的成骨分化
[J]. 中国组织工程研究, 2014, 18(06): 835-840.

[19] Liu L, Guo Y, Chen X, 等. Three-dimensional dynamic culture of pre-osteoblasts seeded in ha-cs/col/nhap composite scaffolds and treated with alpha-zal
[J]. Acta Biochim Biophys Sin (Shanghai), 2012, 44(8): 669-677.

[20] Rizzoli R, Biver E, Brennan-Speranza TC. Nutritional intake and bone health
[J]. Lancet Diabetes Endocrinol, 2021, 9(9): 606-621.

[21] 徐丹, 郭苗苗, 张芸, 等. 纳米制剂技术在骨靶向给药系统中的应用进展
[J]. 中国药师, 2012, 15(02): 180-182+264.

引用本文

彭家伟, 史小方, 宋存峰, 周宁, 朱翔. 荞麦玉米丸纳米制剂对骨质疏松大鼠的改善作用研究 [J]. 国际中医药研究. 2025; 5; (4). 33 - 39.

文献评论

相关学者

相关机构