Journal of International Psychiatry

【Abstract】Objective: To explore the effect of Nesfatin-1 on emotion、behavior and the mechanism in mouse gastric cancer model. Methods: Eighteen 615 mice subcutaneous transplantation tumor animal model of mouse fore-stomach carcinoma cell (MFC) were established.5 days later, the tumor-bearing mice were randomly divided into saline solution control group(NSG), low-dose Nesfatin-1（10ug/kg）(NESLD) and high-dose Nesfatin-1 group（20ug/kg）(NESHD), continuous caudal vein injection for 7 days. Ａ comparion was done among three groups with regard to food intake, body weight gain and tumor weight. The behavior of mice was evaluated by an open field test(OFT)， forced swimming test (FST)，and sucrose preference test(SPT). The concentration of plasma Nesfatin-1 were measured with an enzyme-linked immunosorbent assay (ELISA)，and the level of brain derived neurotrophic factor (BDNF) in Hippocampus were tested by western blot. Results: There was no significant difference in food intake, weight gain and tumor weight among the three groups.Compared with NSG group,NESLD and NESHD group had smaller horizontal moving distance and fewer standing times than NSG group(P<0.05). NESHG spent longer time being immobile in FST than NSG group, while all of these differences were statistically significant( P＜0.05). The level of plasma Nesfatin-1 in NESHD group was significantly higher than that of NSG group , Hippocampus BDNF in NESHD group was significantly lower than that of NSG group (P < 0.05).Conclution: Intravenous injection of Nesfatin-1 could induce depressive-like behavior in mice with gastric cancer, and could reduce the BDNF level in the hippocampus, but had no affection on food intake, body weight, tumor weight.

Nesfatin-1；Gastric cancer；BDNF；Emotion; Behavior