Journal of International Psychiatry

[Abstract] Objective To study the relationship between SLC25A12 gene polymorphism and autistic spectrum disorders. Methods 50 cases of children with autism (100 parents) from May 2015 to May 2017 in our hospital were randomly selected, the genomic DNA was extracted by conventional phenol / chloroform / ethanol, SNP was seen in the crowd out of the NCBI dbSNP database was selected, and was designed to expand the primer on SNP analysis PCR amplification and SSCP, and 2 of SLC25A12 gene SNPs genotype and allele frequency, 2 SNPSs alleles were analyzed. Results The results of SSCP analysis showed that the bands displayed different genotypes, and the SLC25A12 gene decreased at the temperature of 4 C, showing the specific bands of genotype. Hardy Weinberg balance test the distribution of 2 SNP in 50 cases of autism family genotype and allele, Rs3770448 genotype and allele distribution and Hardy Weinberg equilibrium (P>0.05); mutual transmission disequilibrium test results showed that 2 SNPs were linkage disequilibrium of SLC25A12 gene in 50 cases of autism in the family the Rs3770448 and autism candidate loci in linkage, heterozygous parents transfer to the sick children of the pathogenic allele frequencies did not deviate 50%. Conclusion There is no correlation between the polymorphism of SLC25A12 gene and autistic spectrum disorders. It is necessary for the relevant medical scholars to further study the etiological genes of autism.

[Key words] SLC25A12 gene polymorphism; Childhood autism; Relationship