新生儿坏死性小肠结肠炎动物模型的相关进展-国际临床研究杂志-CSCIED科技核心评价数据库-手机版

新生儿坏死性小肠结肠炎动物模型的相关进展

Advances in animal models of neonatal necrotizing enterocolitis

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DOI	10.12208/j.ijcr.20250235
刊名	国际临床研究杂志 International Journal of Clinical Research
年，卷(期)	2025, 9(5)
作者	刘群鹏,杨广承*,
作者单位	承德医学院附属医院河北承德
摘要	坏死性小肠结肠炎（NEC）是早产儿中一种极具破坏性的严重疾病，也是新生儿重症监护病房（NICU）中最为常见的肠道紧急状况之一。NEC不仅对患儿的生存构成直接威胁，还可能导致长期的健康问题。为了更好地理解NEC的病因、发病机制以及病理生理学变化，并探索有效的预防和治疗策略，研究者们在过去五十多年中开发了多种动物模型，如大鼠、小鼠和仔猪。这些模型为NEC的研究提供了重要工具，但每种模型均有其独特的优势和局限性。因此，本文将对NEC模型的开发历程、应用特点及其在研究中的价值进行详细综述，以期为未来的研究方向提供参考。
Abstract	Necrotizing Enterocolitis (NEC) is a highly destructive and severe disease predominantly affecting preterm infants and represents one of the most common gastrointestinal emergencies in Neonatal Intensive Care Units (NICUs). NEC poses a direct threat to the survival of affected infants and can lead to long-term health complications. To enhance our understanding of the etiology, pathogenesis, and pathophysiological changes associated with NEC and to explore effective preventive and therapeutic strategies, researchers have developed a variety of animal models over the past five decades, including rats, mice, and piglets. These models have provided essential tools for NEC research, each with its unique strengths and limitations. Therefore, this review aims to provide a comprehensive overview of the development, application characteristics, and research value of NEC models, offering insights for future research directions.
关键词	坏死性小肠结肠炎；大鼠；小鼠；动物模型
KeyWord	NEC; Rat; Mice; Animal model
基金项目
页码	58-61

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[1] 黄兰,熊涛,唐军,等.新生儿坏死性小肠结肠炎临床诊疗指南(2020)
[J].中国当代儿科杂志,2021,23(01):1-11.
[2] Kaplina A, Kononova S, Zaikova E, Pervunina T, Petrova N, Sitkin S. Necrotizing Enterocolitis: The Role of Hypoxia, Gut Microbiome, and Microbial Metabolites. Int J Mol Sci. 2023 Jan 27;24(3):2471.
[3] Sulistyo A, et al. Animal models of necrotizing enterocolitis: review of the literature and state of the art. Innov Surg Sci. 2018;3(2):87-92.
[4] Barlow B, Santulli TV, Heird WC, Pitt J, Blanc WA, Schullinger JN. An experimental study of acute neonatal enterocolitis-the importance of breast milk. J Pediatr Surg . 1974;IX(5) .
[5] Caplan MS, Hedlund E, Adler L, Hsueh W. Role of asphyxia and feeding in a neonatal rat model of necrotizing enterocolitis. Pediatr Pathol. 1994;14(6):1017-1028.
[6] Zani A, Eaton S, Leon FF, et al. Captopril reduces the severity of bowel damage in a neonatal rat model of necrotizing enterocolitis. J Pediatr Surg. 2008;43(2):308-314.
[7] Wolski M. Modification of Experimental Model of Necrotizing Enterocolitis (NEC) in Rat Pups by Single Exposure to Hypothermia and Hypoxia and Impact of Mother’s Milk on Incidence of Disease. Med. Sci. Monit. 2024;30:e943443.
[8] Krasna IH, Howell C, Vega A, Ziegler M, Evertt Koop C. A mouse model for the study of necrotizing enterocolitis. J Pediatr Surg . 1986;21(1):26–29.
[9] illing T, Simon D , Lu J ,et al. The Roles of Bacteria and TLR4 in Rat and Murine Models of Necrotizing Enterocolitis
[J]. Journal of Immunology, 2006, 177(5):3273-82.
[10] Zhang C, Sherman MP, Prince LS et al. Paneth cell ablation in the presence of Klebsiella pneumoniae induces necrotizing enterocolitis (NEC)-like injury in the small intestine of immature mice. Dis Model Mech 2012;5:522–532
[11] Ginzel M, Feng X, Kuebler JF et al. Dextran sodium sulfate (DSS) induces necrotizing enterocolitis-like lesions in neonatal mice. PLoS One 2017);12:e0182732.
[12] 徐铃琪,马淑蓉,陈璐璐,等. 坏死性小肠结肠炎动物模型建立方法的改进与评价
[J]. 临床小儿外科杂志,2023, 22 (06): 569-575.
[13] Ghoneim N, et al. Delayed initiation but not gradual advancement of enteral formula feeding reduces the incidence of necrotizing enterocolitis (NEC) in preterm pigs. PLoS one. 2014;9:e106888.
[14] Gonzalez LM, Moeser AJ, Blikslager AT. Porcine models of digestive disease: The future of large animal translational research. Transl. Res. 2015;166:12–27.
[15] Sibbons P, Spitz L, et al. Relationship of birth weight to the pathogenesis of necrotizing enterocolitis in the neonatal piglet. Pediatr Pathol. 1988;8:151–162.
[16] Cohen IT, Nelson SD, Moxley RA, Hirsh MP, Counihan TC, Martin RF. Necrotizing enterocolitis in a neonatal piglet model. J Pediatr Surg . 1991;26(5):598–601.
[17] Sangild PT, Siggers RH, et al. Diet- and colonization-dependent intestinal dysfunction predisposes to necrotizing enterocolitis in preterm pigs. Gastroenterology. 2006;130: 1776–1792.
[18] Good M, Sodhi CP, Ozolek JA, Buck RH, Goehring KC, Thomas DL, et al. Lactobacillus rhamnosus HN001 decreases the severity of necrotizing enterocolitis in neonatal mice and preterm piglets: evidence in mice for a role of TLR9. Am J Physiol Gastrointest Liver Physiol. (2014) 306:G1021–1032.
[19] Ragan MV, Wala SJ, Sajankila N, et al. Development of a novel definitive scoring system for an enteral feed-only model of necrotizing enterocolitis in piglets. Front Pediatr. 2023;11: 1126552.
[20] Clark DA, Thompson JE, Weiner LB, McMillan JA, Schneider AJ, Rokahr JE. Necrotizing enterocolitis: intraluminal biochemistry in human neonates and a rabbit model. Pediatr Res . 1985;19(9):919–921.
[21] Miller MJ, McNeill H, Mullane KM, et al. SOD prevents damage and attenuates eicosanoid release in a rabbit model of necrotizing enterocolitis. Am J Physiol Gastrointest Liver Physiol 1988;255:G556–65.
[22] Gurien LA, Stallings-Archer K, Smith SD. Probiotic Lactococcus lactis decreases incidence and severity of necrotizing enterocolitis in a preterm animal model. J Neonatal Perinatal Med 2018;11:65–9.

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刘群鹏,杨广承*. 新生儿坏死性小肠结肠炎动物模型的相关进展 [J]. 国际临床研究杂志. 2025; 9; (5). 58 - 61.

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