International Medical Research Frontier

A headspace gas chromatography method for the determination of ethanol in kanamycin sulfate was established, and the external standard method was used for the determination. The specificity, linearity, repeatability, accuracy, detection limit and quantification limit of the analytical method were verified. The results show that this method has good specificity, good linear relationship within the range (r=0.9997), the repeatability RSD is 0.75%, the average recovery rate of accuracy is 93.9%, the RSD is 2.3%, the detection limit and quantification limit are 0.01, respectively µg/mL and 0.05µg/mL, which are equivalent to 0.02ppm and 0.1ppm of ethanol in the samples, respectively. The method is simple and sensitive, and can be used for the quantitative determination of ethanol residues in kanamycin sulfate.

Kanamycin; Ethanol; Headspace gas chromatography