Comparative Evaluation of Soybean Genotypes for In Vitro Regeneration via Direct Organogenesis-Crop Breeding, Genetics and Genomics-CSCIED科技核心评价数据库-手机版

Comparative Evaluation of Soybean Genotypes for In Vitro Regeneration via Direct Organogenesis

Comparative Evaluation of Soybean Genotypes for In Vitro Regeneration via Direct Organogenesis

ES评分 0 浏览量：107 下载量：1

DOI	10.20900/cbgg20250011
刊名	Crop Breeding, Genetics and Genomics CBGG
年，卷(期)	2025, 7(3)
作者	Atiqa Rehman*,Muhammad Ammar Rafique,Zaheer Ahmed,
作者单位	Horticulture & Natural Resources, Kansas State University, Manhattan, KS 66506, USA ; Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Ghulam Muhammad Abad 38000, Pakistan ;
摘要	Genetic transformation plays a vital role in enhancing crop performance under changing climatic conditions. However, soybean (Glycine max L.) remains a transformation-recalcitrant species, with regeneration efficiency strongly influenced by genotype, explant type, and in vitro culture conditions. In this study, four soybean genotypes: Faisal Soybean, AARI Soybean, Rawal-I, and Williams-82 were evaluated for their regeneration potential via direct organogenesis using half-split cotyledonary explants. Explants were cultured on Murashige and Skoog (MS) medium supplemented with varying concentrations of benzyl amino purine (BAP) and naphthalene acetic acid (NAA) for shoot induction, and indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) for root induction. Among the tested genotypes, Faisal Soybean exhibited the highest regeneration efficiency, with 40.13% shoot induction and 26.83% root induction, followed by AARI Soybean. The optimal shoot induction was achieved on MS medium containing 2.0 mg·L−1 BAP + 0.1 mg·L−1 NAA, while the highest root induction was observed on medium with 0.5 mg·L−1 IBA + 1.2 mg·L−1 IAA. In contrast, Rawal-I showed the lowest regeneration response across all stages. These findings highlight significant genotype-dependent variation in regeneration potential, reinforcing the importance of selecting responsive cultivars for transformation. The optimized protocol developed in this study provides a reliable foundation for future genetic transformation and crop improvement efforts in soybean.
Abstract	Genetic transformation plays a vital role in enhancing crop performance under changing climatic conditions. However, soybean (Glycine max L.) remains a transformation-recalcitrant species, with regeneration efficiency strongly influenced by genotype, explant type, and in vitro culture conditions. In this study, four soybean genotypes: Faisal Soybean, AARI Soybean, Rawal-I, and Williams-82 were evaluated for their regeneration potential via direct organogenesis using half-split cotyledonary explants. Explants were cultured on Murashige and Skoog (MS) medium supplemented with varying concentrations of benzyl amino purine (BAP) and naphthalene acetic acid (NAA) for shoot induction, and indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) for root induction. Among the tested genotypes, Faisal Soybean exhibited the highest regeneration efficiency, with 40.13% shoot induction and 26.83% root induction, followed by AARI Soybean. The optimal shoot induction was achieved on MS medium containing 2.0 mg·L−1 BAP + 0.1 mg·L−1 NAA, while the highest root induction was observed on medium with 0.5 mg·L−1 IBA + 1.2 mg·L−1 IAA. In contrast, Rawal-I showed the lowest regeneration response across all stages. These findings highlight significant genotype-dependent variation in regeneration potential, reinforcing the importance of selecting responsive cultivars for transformation. The optimized protocol developed in this study provides a reliable foundation for future genetic transformation and crop improvement efforts in soybean.
关键词	direct organogenesis; soybean (Glycine max); benzyl amino purine (BAP); naphthalene acetic acid (NAA); indole butyric acid (IBA); indole acetic acid (IAA); plant regeneration; tissue culture
KeyWord	direct organogenesis; soybean (Glycine max); benzyl amino purine (BAP); naphthalene acetic acid (NAA); indole butyric acid (IBA); indole acetic acid (IAA); plant regeneration; tissue culture
基金项目
页码	-

参考文献
相关文献

1.Li Y, Guan R, Liu Z, Ma Y, Wang L, Li L, et al. Genetic structure and diversity of cultivated soybean (Glycine max (L.) Merr.) landraces in China. Theor Appl Genet. 2008;117(6):857-71.
2.Department of Agriculture. Project Director’s Report, AICRP on Soybean, DOR. Indore (India): Department of Agriculture; 2011.
3.Messina MJ. Legumes and soybeans: Overview of their nutritional profiles and health effects. Am J Clin Nutr. 1999;70(3 Suppl):439S-50S.
4.ISAAA. Biotechnology to Improve Hybrid Breeding of Soybeans. Crop Biotech Update. 2023 Aug 30. Available from: https://www.isaaa.org/kc/cropbiotechupdate/article/default.asp?ID=20379. Accessed on 17 Jul 2025.
5.Manavalan LP, Guttikonda SK, Tran L-SP, Nguyen HT. Physiological and molecular approaches to improve drought resistance in soybean. Plant Cell Physiol. 2009;50(7):1260-76.
6.Roy ID, Kumari N, Pandi G, Singh RK, Shirasawa K, Himabindu K, et al. CRISPR/Cas9-mediated targeted mutagenesis of GmGOLS3 enhances drought tolerance in soybean. Front Plant Sci. 2022;13:900318. Available from: https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.900318/full. Accessed on 17 Jul 2025.
7.Han Y, Tian L, VanBuren R, Yan A, Schnable JC. Widespread and functional plant alternative splicing at single-cell resolution. Cell Genom. 2024;6(6):100417. Available from: https://www.sciencedirect.com/science/article/pii/S2590346224004176. Accessed on 17 Jul 2025.
8.Ullah MW, Ali S, Khan A, Ahmed MA, Noon RK, Khan AH, et al. Impacts of climate change on rice yield and adaptability in Punjab, Pakistan. Plants (Basel). 2024;13(21):3073. Available from: https://www.mdpi.com/2223-7747/13/21/3073. Accessed on 17 Jul 2025.
9.Boachon B, Burdziej A, Kaminski S, Kundu A, Miesch L, Guirimand G, et al. CYP76 family members in basil camphor biosynthesis: Functional identification and evolution insight. Front Plant Sci. 2017;8:246. Available from: https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.00246/full. Accessed on 17 Jul 2025.
10.Kaur G, Singh A, Singh I, Sharma P. Nitrogen use efficiency and management in Indian agriculture: A review. J Integr Agric. 2017;16(12):2823-37. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC5485301/. Accessed on 17 Jul 2025.
11.Buttar GS, Ahmad M, Rahman H, Bakhsh A, Rehman S, Muhammad D, et al. Climatic ideotype: A possible solution for adaptation of soybean under Punjab, Pakistan climate. Int J Hydrol. 2018;2(6):495-9. Available from: https://medcraveonline.com/IJH/climatic-ideotype-a-possible-solution-for-adaptation-of-soybean-under-punjab-pakistan-climate.html. Accessed on 17 Jul 2025.
12.PR Newswire. Friends afar: How do Chinese Scientists Grow Soybeans in Pakistan?
[Internet]. 2024. Available from: https://www.prnewswire.com/news-releases/friends-afar-how-do-chinese-scientists-grow-soybeans-in-pakistan-302407835.html. Accessed on 17 Jul 2025.
13.Sairam RV, Franklin G, Hassel R, Smith B, Meeker K, Kashikar N, et al. A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus. Plant Cell Tissue Organ Cult. 2003;75:79-85.
14.Mante S, Scorza R, Cordts J. A simple, rapid protocol for adventitious shoot development from mature cotyledons of Glycine max cv. Bragg. In Vitro Cell Dev Biol. 1989;25:385-8.
15.Ma XH, Wu TL. Rapid and efficient regeneration in soybean
[Glycine max (L.) Merrill] from whole cotyledonary node explants. Acta Physiol Plant. 2008;30(2):251-6.
16.Tripathi M, Tiwari S. Epigenesis and high frequency plant regeneration from hypocotyl explants of soybean (Glycine max L. Merrill). Plant Tissue Cult. 2003;13(1):61-73.
17.Yoshida T. Adventitious shoot formation from hypocotyl sections of mature soybean seeds. Breed Sci. 2002;52(1):1-8.
18.Dan Y, Reichert NA. Organogenic regeneration of soybean from hypocotyl explants. In Vitro Cell Dev Biol Plant. 1998;34(1):14-21.
19.Wright MS, Ward DV, Hinchee MA, Carnes MG, Kaufman RJ. Plant regeneration from epicotyl and primary leaf explants. Plant Cell Rep. 1987;6(2):83-9.
20.Kim J, LaMotte CE, Hack E. Plant regeneration in vitro from primary leaf nodes of soybean (Glycine max) seedlings. J Plant Physiol. 1990;136(5):664-8. doi: 10.1016/S0176-1617(11)81341-6
21.Raza G, Singh MB, Bhalla PL. In vitro plant regeneration from commercial cultivars of soybean. Int J Genomics. 2017;2017:7379693.
22.Franklin G, Trieu TN, Dixon RA. Factors affecting regeneration in legumes: A comprehensive review. Plant Cell Tissue Organ Cult. 2004;79(1):1-12.
23.Yildiz M, Er C, Mavituna F. Environmental and genotype effects on plant regeneration. Plant Cell Tissue Organ Cult. 2002;71(3):175-82.
24.Bailey MA, Boerma HR, Parrott WA. Genotype effects on proliferative embryogenesis and plant regeneration of soybean. In Vitro Cell Dev Biol Plant. 1993;29(2):102-8.
25.Asad SA, Wahid MA, Shaheen F, Raza A, Farooq M. Soybean production in Pakistan: Experiences, challenges and prospects. Int J Agric Biol. 2020;24(4):995-1005. doi: 10.17957/IJAB/15.1526.
26.Malik MFA, Ashraf M, Qureshi AS, Ghafoor A. Assessment of genetic variability, correlation and path analyses for yield and its components in soybean
[Glycine max (L.) Merrill]. J Agric Res. 2007;45(3):187-92.
27.Aziz MA, Oyatomi OA, Oladosu Y, Adetula OA, Popoola JO. Genetic diversity and population structure of soybean (Glycine max (L.) Merril) accessions in West Africa. PLoS ONE. 2021;16(5):e0312079.
28.Wang S, Bai Y, Shen C, Wu Y, Zhang S, Jiang D, et al. Auxin-related gene families in soybean and their hormone-responsive expression profiles. Front Plant Sci. 2020;11:1228. Available from: https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.01228/full. Accessed on 17 Jul 2025.
29.Shen K, Yang H, Li X, Wu Y, Zhou J. Genome-wide association analysis reveals the genetic mechanisms of soybean resistance to Fusarium oxysporum. Int J Mol Sci. 2022;23(14):8137. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC9231586/. Accessed on 17 Jul 2025.
30.Bai Y, Wang S, Yang Q, Zhang X, Sun R, Jiang D, et al. The evolving landscape of the soybean genome: Insights into structure and domestication. bioRxiv
[Preprint]. 2024 Apr 22. Available from: https://www.biorxiv.org/content/10.1101/2024.04.19.590356v1.full-text. Accessed on 17 Jul 2025.
31.Kantayos V, Chang-Hyu Bae CH. Optimized shoot induction and histological study of in-vitro cultured Korean soybean cultivars. Vietnam J Agric Sci. 2019;7(2):2121-34.
32.Li X, Han Y, Wei Y, Acharya A, Farmer AD, Zhao X, et al. Analysis of soybean somatic embryogenesis using chromosome segment substitution lines and transcriptome sequencing. BMC Genomics. 2019;20:729. doi: 10.1186/s12864-019-6107-8
33.Zhang Z, Xing A, Staswick P, Clemente TE. Efficient regeneration from cotyledonary nodes in soybean. Plant Cell Tissue Organ Cult. 2011;106(3):275-84.
34.Dang W, Wei ZM. High frequency plant regeneration from the cotyledonary node of common bean. Biol Plant. 2009;53(2):312-6.
35.Siddique I, Anis M. Thidiazuron induced high frequency shoot bud formation and plant regeneration from cotyledonary node explants of Capsicum annuum L. Biol Plant. 2006;50(4):701-4. doi: 10.1007/s10535-006-0114-1
36.Jeyakumar M, Jayabalan N. In vitro plant regeneration from cotyledonary node of Psoralea corylifolia L. Plant Tissue Cult. 2002;12:125-9.
37.Liu X, Jin J, Wang G, Song Q, Chen Q, Chen Q, et al. Highly efficient shoot regeneration from cotyledonary nodes of vegetable soybean. Korean J Hortic Sci Technol. 2010;28(2):307-13.
38.Park SH, Lee BM, Salas MG, Srivatanakul M, Smith RH. Efficient and genotype-independent Agrobacterium-mediated transformation of soybean using a cotyledonary node method. Plant Cell Rep. 2004;23(5):386-90.
39.Paz MM, Shou H, Guo Z, Zhang Z, Banerjee AK, Wang K. Optimization of Agrobacterium-mediated transformation of soybean. Plant Cell Rep. 2004;22(1):61-8.
40.Radhakrishnan R, Ranjithakumari BD. Callus induction and plant regeneration of Indian soybean (Glycine max (L.) Merr. cv. CO3) via half seed explant culture. J Agric Technol. 2007;3(2):287-97.
41.Verma K, Rani A, Saini R. An efficient plant regeneration system from half seed explants of soybean
[Glycine max (L.) Merrill] using thidiazuron. Soybean Res. 2011;8:62-8.
42.Biabani A, Hashemi M, Herbert, SJ. Agronomic performance of two intercropped soybean cultivars. Int J Plant Prod. 2012;2(3):215-22.

引用本文

Atiqa Rehman*,Muhammad Ammar Rafique,Zaheer Ahmed. Comparative Evaluation of Soybean Genotypes for In Vitro Regeneration via Direct Organogenesis [J]. Crop Breeding, Genetics and Genomics. 2025; 7; (3). - .

文献评论

相关学者

相关机构