壳聚糖与γ-聚谷氨酸对三种海洋微藻的絮凝收获作用研究-湖南生态科学学报-CSCIED科技核心评价数据库-手机版

壳聚糖与γ-聚谷氨酸对三种海洋微藻的絮凝收获作用研究

Study on Harvesting Three Species of Marine Microalgae via Flocculation Using Chitosan and γ-Polyglutamic Acid

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DOI	10.3969/j.issn.2095-7300.2025.01.009
刊名	湖南生态科学学报 Journal of Hunan Ecological Science
年，卷(期)	2025, 12(1)
作者	潘凯琳,郑怡鸿,赖俊翔,李智巧,姜发军*(,
作者单位	广西大学资源环境与材料学院,广西南宁; 深圳大学生命与海洋科学学院,广东深圳; 广西科学院,广西海洋科学院(广西红树林研究中心),广西近海海洋环境科学重点实验室,广西南宁; 广西科学院防城港分院(北部湾海洋产业研究院),广西防城港
摘要	【目的】本研究旨在评估壳聚糖(CTS)与γ-聚谷氨酸(PGA)作为絮凝剂对海水小球藻、杜氏盐藻、紫球藻三种海洋微藻的絮凝收获效果,并探究不同絮凝剂用量及投加次序对絮凝效率的影响,以期为开发环保高效的微藻收获技术提供参考。【方法】本研究采用CTS和PGA作为絮凝剂,对海水小球藻、杜氏盐藻、紫球藻三种海洋微藻进行絮凝实验。实验包括CTS单独使用以及联合PGA的复合使用,通过调整絮凝剂用量及投加次序,观察并记录其对絮凝效果的影响。【结果】对于海水小球藻和杜氏盐藻,CTS与PGA的联合使用显著增强了絮凝效果。其中,先投加CTS再投加PGA的复合方式表现最佳,海水小球藻的絮凝率高达96.62%,盐藻的絮凝率则达到87.54%。对于盐藻,在最佳投加次序下,CTS的最佳作用浓度为150mg/L(絮凝率为78.45%),PGA的最佳作用浓度为75mg/L(絮凝率为68.92%)。对于紫球藻,CTS与PGA的联合使用及CTS单独使用均能有效提高其絮凝效果。其中,CTS单独作用时效果最佳,最佳作用浓度为150mg/L(絮凝率为99.18%)。【结论】本研究表明,采用先投加CTS再投加PGA的联合使用策略,能有效提高三种海洋微藻的絮凝效果。这一发现为开发环保高效的微藻收获技术提供了有价值的参考,并为未来的研究提供了方向。
Abstract	【Objective】The aim of this study was to evaluate the effect of chitosan (CTS) and γ-polyglu- tamic acid ( PGA) as flocculants on the harvesting of three kinds of marine microalgae, namely, Chlorella sp. , Dunaliella salina, and Porphyridium cruentum, and to investigate the effects of different flocculant dosage and accession sequences on the flocculation efficiency with the aim of providing a refer- ence to the development of environmentally friendly and efficient microalgae harvesting technology. 【Method】In this study, CTS and PGA were used as flocculants to flocculate three kinds of marine mi- croalgae, namely Chlorella sp. , D. salina, and P. cruentum. The experiments included the use of CTS alone and the combined use of PGA and CTS, and the effects on the flocculation effect were explored by adjusting the dosage of flocculants and the order of dosing. 【Result】For Chlorella sp. and D. salina, the combined use of CTS and PGA significantly enhanced the flocculation effect. Among them, the combina- tion of CTS followed by PGA showed the best performance in the experiment of different sequences of flocculants, with the flocculation rate of Chlorella sp. reaching 96. 62% and that of D. salina reaching 87. 54% . For D. salina, under the optimal dosing sequence, the optimal concentration of CTS was 150 mg / L (flocculation rate of 78. 45% ), and the optimal concentration of PGA was 75 mg / L (floccula- tion rate of 68. 92% ). In the case of P. cruentum, both the combined use of CTS and PGA and the use of CTS alone could effectively improve the flocculation effect. Among them, the best effect was achieved when CTS was used alone, and the optimum concentration of CTS was 150 mg / L (flocculation rate of 99. 18% ). 【Conclusion】This study showed that the combined use strategy of CTS followed by PGA can effectively improve the flocculation effect of Chlorella sp. , D. salina; as to P. cruentum, the best har- vesting can achieve in the case of CTS used alone. This finding provided a valuable reference for the de- velopment of environmentally friendly and efficient microalgae harvesting technology and provided a direc- tion for further research.
关键词	壳聚糖;γ-聚谷氨酸;絮凝;双絮凝剂;微藻收获
KeyWord	chitosan ( CTS ); γ-polyglutamic acid ( PGA ); flocculation; dual flocculants; microalgae harvesting
基金项目
页码	74-80

参考文献
相关文献

[1] MOLINA GRIMA E,BELARBI E H,ACIéN FERNáNDEZ F G,et al. Recovery of microalgal biomass and metabolites: Process options and economics
[J]. Biotechnology Advances, 2003,20(7 / 8):491-515.
[2] 王博,许胜超,李雪霏,等. 微藻提取脂质制取生物柴油的研究进展
[J]. 化工新型材料,2021,49(5):21-25.
[3] DEMIRBAS A. Use of algae as biofuel sources
[J]. Energy Conversion and Management,2010,51(12):2738-2749.
[4] APONE F,BARBULOVA A,COLUCCI M G. Plant and mi- croalgae derived peptides are advantageously employed as bioactive compounds in cosmetics
[ J]. Frontiers in Plant Science,2019,10:756.
[5] BARKIA I,SAARI N,MANNING S R. Microalgae for high- value products towards human health and nutrition
[J]. Ma- rine Drugs,2019,17(5):304.
[6] DRAAISMA R B,WIJFFELS R H,SLEGERS P M,et al. Food commodities from microalgae
[J]. Current Opinion in Biotechnology,2013,24(2):169-177.
[7] ALLEN K M,HABTE-TSION H M,THOMPSON K R,et al. Freshwater microalgae (Schizochytrium sp. ) as a substi- tute to fish oil for shrimp feed
[J]. Scientific Reports,2019, 9:6178.
[8] CHEN G Y,ZHAO L,QI Y,et al. Chitosan and its deriva- tives applied in harvesting microalgae for biodiesel produc- tion:An outlook
[J]. Journal of Nanomaterials,2014,2014:3.
[9] ENAMALA M K,ENAMALA S,CHAVALI M,et al. Pro- duction of biofuels from microalgae—A review on cultivation, harvesting,lipid extraction,and numerous applications of mi- croalgae
[J]. Renewable and Sustainable Energy Reviews, 2018,94:49-68.
[10] KO W C,CHANG C K,WANG H J,et al. Process optimi- zation of microencapsulation of curcumin in γ-polyglutamic acid using response surface methodology
[J]. Food Chem- istry,2015,172:497-503.
[11] RASHID N,REHMAN S U,HAN J I. Rapid harvesting of freshwater microalgae using chitosan
[ J]. Process Bio- chemistry,2013,48(7):1107-1110.
[12] 冯闪闪,吴幸强,王纯波,等. 阳离子淀粉制备条件优化及其对野外蓝藻的絮凝效果
[ J]. 环境科学与技术, 2018,41(5):37-42.
[13] 张海阳,刘春华,匡亚莉,等. 基于泡沫浮选的能源微藻采收实验研究
[J]. 可再生能源,2016,34(2):268-273.
[14] CUI J S,NIU X J,ZHANG D Q,et al. The novel chitosan- amphoteric starch dual flocculants for enhanced removal of Microcystis aeruginosa and algal organic matter
[J]. Carbo- hydrate Polymers,2023,304:120474.
[15] TAGHAVIJELOUDAR M,YAQOUBNEJAD P,AHANGAR A K,et al. A rapid, efficient and eco-friendly approach for simultaneous biomass harvesting and bioproducts extraction from microalgae:Dual flocculation between cationic surfac- tants and bio-polymer
[ J]. Science of the Total Environ- ment,2023,854:158717.
[16] LIANG C L,YANG Y R,XIA Y Q,et al. The optimization of Chlorella vulgaris flocculation harvesting by chitosan and calcium hydroxide
[J]. Indian Journal of Microbiology,2022, 62(2):266-272.
[17] YIN Z H,LI S X,HU D,et al. Performance evaluation of different chitosan-clay composite materials for efficient harvesting of Chlorella vulgaris and impacts on downstream bioproduct processing and water reusability
[J]. Chemical Engineering Journal,2022,430:132892.
[18] FERNANDES A R A C,SGANZERLA W G,ALVES GRA- NADO N P,et al. Implication of organic solvents in the pre- cipitation of γ-polyglutamic acid for application as a sus- tainable flocculating agent
[J]. Biocatalysis and Agricultural Biotechnology,2023,50:102698.
[19] SAMAL S,BANERJEE S,DEY P,et al. Production and characterization of a novel poly amino acid from a thermoph- ilic bacterium,and preliminary testing of its coagulating po- tential for imminent wastewater treatment application
[J]. International Journal of Biological Macromolecules,2023, 246:125589.
[20] 牛雅倩,李雨桐,赵娟,等. 聚谷氨酸去除马铃薯废水蛋白质及絮凝机理初探
[ J]. 食品与发酵工业,2021, 47(21):178-184.
[21] 石从众,王成,刘清涛,等. 高分子复合絮凝剂在废水处理中的研究进展
[J]. 毛纺科技,2023,51(7):104-110.
[22] LI M, ZHU X Q, YANG H, et al. Treatment of potato starch wastewater by dual natural flocculants of chitosan and poly-glutamic acid
[J]. Journal of Cleaner Production, 2020,264:121641.
[23] 熊鹰,张亚蒙,余关龙,等. 复合絮凝剂处理高浓度果胶废水的性能研究
[J]. 水处理技术,2022,48(11):98- 101,107.
[24] MA J,WANG R N,WANG X Y,et al. Drinking water treat- ment by stepwise flocculation using polysilicate aluminum magnesium and cationic polyacrylamide
[J]. Journal of En- vironmental Chemical Engineering,2019,7(3):103049.
[25] CAI Q J,GONG S H,SONG K Z,et al. Effective harvesting of Scenedesmus using quaternary ammonium chitosan and xanthan gum:Formation of mega flocs with oppositely charged polyelectrolytes
[ J]. Journal of Cleaner Production,2021, 329:129730.
[26] ZHANG Y L,LI M,ZHANG G H,et al. Efficient treatment of the starch wastewater by enhanced flocculation—coagu- lation of environmentally benign materials
[J]. Separation and Purification Technology,2023,307:122788.
[27] ENDRAWATI H,WIDIANINGSIH W,NURAINI R,et al. The effect of chitosan concentration on flocculation efficiency microalgae Porphyridium cruentum (Rhodhophyta)
[J]. IOP Conference Series:Earth and Environmental Science,2021, 919(1):012052.
[28] NADIIRUL Z,NABILA S N,JOHAR M,et al. Harvesting Aurantiochytrium sp. SW1 via flocculation using chitosan: Effects of flocculation parameters on flocculation efficiency and Zeta potential
[J]. Marine Drugs,2023,21(4):251.
[29] ELCIK H,KARADAG D,KARA A I,et al. Microalgae bio- mass harvesting using chitosan flocculant:Optimization of operating parameters by response surface methodology
[J]. Separations,2023,10(9):507.

引用本文

潘凯琳,郑怡鸿,赖俊翔,李智巧,姜发军*(. 壳聚糖与γ-聚谷氨酸对三种海洋微藻的絮凝收获作用研究 [J]. 湖南生态科学学报. 2025; 12; (1). 74 - 80.

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