Journal of Hunan Ecological Science

【Objective】It is to improve the reproductive efficiency of Hydrangea macrophylla ‘Blue Mama’ by establishing an efficient, stable and rapid propagation system. 【Method】The effects of different concentrations of plant growth regulators on the germination, proliferation, and rooting of axillary buds and the effect of different substrate ratios on seedling transplantation were investigated using H. macrophylla ‘Blue Mama’ with axillary bud stem segments as explants. 【Result】(1) In the induction culture stage, the induction rate showed a downward trend as the concentrations of 6-BA and NAA in- creased. When 6-BA was 0. 5 mg / L and NAA was 0. 1 mg / L, the highest induction rate (47. 8% ) was obtained. The optimal induction culture medium was MS+0. 5 mg / L 6-BA+0. 1 mg / L NAA. (2) In the proliferation culture stage, the most suitable proliferation medium was MS+2. 0 mg / L 6-BA+0. 2 mg / L NAA, the proliferation rate was positively proportional to the concentration of 6-BA, and the highest pro- liferation coefficient was 3. 16. (3) During the rooting culture stage, with the increase of IBA concentra- tion, the growth condition exhibited a trend of initially ascending and then descending. When the IBA concentration was 0. 4 mg / L, the rooting rate reached 100% (root length was 4. 07 cm and number of main roots was 12. 3). (4) In the transplanting substrate with a volume ratio of 1 ∶1 of vermiculite to perlite, the survival rate was 100% , the plant growth was vigorous, and both the plant height and crown width were higher than those in other treatments. 【Conclusion】A high efficient propagation system for H. macrophylla ‘Blue Mama’ has been established, which provides some theoretical references for the rapid propagation of Hydrangea macrophylla.

Hydrangea macrophylla; stem segments; rapid propagation; transplanting substrate