Journal of Hunan Ecological Science

【Objective】Through the study on the tissue culture and rapid propagation of the bulbs of Hippeastrum vittatum ‘Candy Nymph’, the aseptic culture system was established, to provide a theoreti- cal basis for the efficient breeding of high-quality bulbs of H. vittatum, and lay a foundation for further promoting the industrialization production of H. vittatum. 【Method】The bulbs of H. vittatum ‘ Candy Nymph’ were used as materials, and different disinfection time and culture media were used, to investi- gate the effects of different factors on disinfection, shoot induction, proliferation and rooting of H. vitta- tum ‘Candy Nymph’. 【Result】The disinfecting effects of different disinfecting time on the bulbs of H. vittatum ‘ Candy Nymph’ were different. When the disinfecting time was 60 s with 75% alcohol and 12 min with 2% NaClo, the survival rate of the bulbs was the highest, being 73. 3% ;Among the different media, the induction rate of bulb bud induction medium with MS+1. 0 mg / L 6-BA+1. 5 mg / L NAA+ 2. 0 mg / L TDZ+1 g / L AC was the best, reaching 75. 32% ;MS +2. 0 mg / L 6-BA+1. 5 mg / L NAA+ 1. 0 mg / L TDZ+1 g / L AC was the best medium for proliferation, and the proliferation coefficient was 5. 28; The best rooting medium was MS+0. 5 mg / L IBA+1. 0 mg / L NAA+1 g / L AC, with an average rooting number of 5. 6, an average rooting length of 6. 4 cm and a rooting rate of 96. 42% . 【Conclusion】 The most suitable disinfection method for the sterile culture system of H. vittatum ‘Candy Nymph’ was 75% alcohol disinfection for 60 seconds+2% NaClO solution disinfection for 12 minutes; The optimum medium for bulblet induction was MS+1. 0 mg / L 6-BA+1. 5 mg / L NAA+2. 0 mg / L TDZ+1 g / L AC. The proliferation medium was MS+2. 0 mg / L 6-BA+1. 5 mg / L NAA+1. 0 mg / L TDZ+1 g / L AC, and the rooting medium was MS+0. 5 mg / L IBA+1. 0 mg / L NAA+1 g / L AC.

Hippeastrum vittatum;bulb;induction;tissue culture;plant hormones