类珠藻对硝化抑制剂3,4-二甲基吡唑磷酸盐胁迫的生理响应-湖南生态科学学报-CSCIED科技核心评价数据库-手机版

类珠藻对硝化抑制剂3,4-二甲基吡唑磷酸盐胁迫的生理响应

Physiological Responses of Aliinostoc sp. to Stress from Nitrification Inhibitor 3, 4-Dimethylpyrazole Phosphate

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DOI	10.3969/j.issn.2095-7300.2025.01.003
刊名	湖南生态科学学报 Journal of Hunan Ecological Science
年，卷(期)	2025, 12(1)
作者	张润钊,邵继海*,洪美灵,李好,
作者单位	湖南农业大学环境与生态学院,湖南长沙
摘要	【背景】硝化抑制剂能抑制土壤微生物的硝化过程,提高氮肥利用率和减缓硝化作用引起的土壤酸化,但其对非硝化微生物的影响尚不明确。【目的】探明稻田固氮蓝藻———类珠藻(Aliinostocsp.YYLX235)对不同浓度3,4-二甲基吡唑磷酸盐(DMPP)的生理响应,以期为二者在稻田中的合理使用提供理论指导。【方法】以类珠藻(Aliinostocsp.YYLX235)为材料,通过对光合色素、细胞活性、抗氧化性及固氮酶活性等指标的测定,评估其对DMPP的生理响应。【结果】浓度为25~100mg/L的DMPP可以显著降低类珠藻YYLX235的细胞活性,抑制该藻的生长;DMPP胁迫能提高YYLX235细胞中叶绿素a和类胡萝卜素的含量,但降低藻蓝蛋白(PC)和别藻蓝蛋白(APC)的含量。类珠藻YYLX235的光合作用和固氮活性对DMPP胁迫响应灵敏,浓度为25mg/L的DMPP可以显著降低YYLX235光系统Ⅱ(PSⅡ)的电子传递效率并抑制该藻的固氮活性;DMPP胁迫导致YYLX235藻细胞内抗氧化酶活性升高,细胞内脂质氧化终产物丙二醛含量增加。【结论】DMPP对类珠藻具有较强的生物毒性,能干扰其光系统Ⅱ电子传递,诱导藻细胞受到氧化性损伤,降低其固氮活性。
Abstract	【Background】Nitrification inhibitor can inhibit the nitrification process of soil microorgan- isms, improve nitrogen utilization rate, and slow down the soil acidification caused by nitrification, but its effect on non-nitrifying microorganisms is still unclear. 【Objective】The physiological responses of Ali- inostoc sp. YYLX235 to different concentrations of 3,4-dimethylpyrazole phosphate (DMPP) were inves- tigated in order to provide theoretical guidance for their rational use in the paddy field. 【Method】The physiological effects of DMPP on Aliinostoc sp. YYLX235 were evaluated by measuring photosynthetic pigment, cell activity, antioxidant activity, nitrogenase activity and so on. 【Result】DMPP with the con- centration of 25-100 mg / L could significantly reduce the cell vitality of YYLX235 and inhibit the growth of this alga. DMPP stress could increase the contents of chlorophyllin a and carotenoids in Aliinostoc sp. YYLX235 cells, but decrease the contents of phycocyanin and allophycocyanin in algal cells. The photo- synthetic and nitrogen-fixing activity of YYLX235 were sensitive to DMPP stress. DMPP with a concen- tration of 25 mg / L could significantly reduce the electron transfer efficiency of photosynthetic system Ⅱ (PSⅡ) and inhibit the nitrogen fixing activity of YYLX235. DMPP stress increased the activity of an- tioxidant enzymes and the content of malondialdehyde, the end product of lipid oxidation, in YYLX235 cells. 【Conclusion】DMPP has strong biotoxicity to YYLX235, which can interfere with the electron transport of PSⅡ, induce oxidative damage to algal cells, and reduce the activity of nitrogen-fixation.
关键词	类珠藻;光合系统;氧化-抗氧化系统;固氮活性
KeyWord	Aliinostoc sp. ; photosynthetic system; oxidation-antioxidant system; nitrogen-fixing activity
基金项目
页码	24-33

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张润钊,邵继海*,洪美灵,李好. 类珠藻对硝化抑制剂3,4-二甲基吡唑磷酸盐胁迫的生理响应 [J]. 湖南生态科学学报. 2025; 12; (1). 24 - 33.

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