Journal of Hunan Ecological Science

【Objective】The aim of this study is to establish a fast breeding system of ‘ Wuyuan- huangju’, and to provide technical support for the large-scale production of ‘ Wuyuanhuangju ’ seedlings. 【Method】The disinfection, primary culture, secondary culture, rooting culture and seedling transplantation process of ‘Wuyuanhuangju’ were studied by using young stem segments of ‘Wuyuan- huangju’ as the explant, hypochloric acid as the disinfectant, the disinfection times were 5 (A1), 10 (A2), 15 (A3), and 20 (A4) minutes, respectively, and different plant growth regulator combinations added with MS culture medium as the basic culture medium. 【Result】Different sterilization time and dif- ferent plant growth regulator ratios had significant effects on the primary culture effect of ‘ Wuyuan- huangju’ explants. The ratios of browning and bacterial contamination of tissue culture seedling in differ- ent treatments caused by sterilization time were: A1A2>A3>A4 respectively; The me- dium supplemented with 6-BA 2. 0 mg / L+NAA 0. 2 mg / L, and KT 0. 1 mg / L had the highest bud induc- tion rate, germination index, and seeding height during the initial cultivation; The medium supplemented with 6-BA 1. 5 mg / L, NAA 0. 2 mg / L, and KT 0. 1 mg / L showed the highest bud induction rate and germination index during subculture; The tissue culture seedlings with added 6-BA 0. 5 mg / L + NAA 0. 3 mg / L+IBA 0. 2 mg / L had the highest rooting rate, the highest average number of roots per plant, and the longest average root length per plant during rooting cultivation; the optimal number of days of seedling refining was 6 ~ 7 days. 【Conclusion】The optimal disinfection method was 70% alcohol disin- fection for 10 seconds, followed by disinfection with 2% hypochloric acid for 10 minutes; the optimal pri- mary culture medium MS+6-BA 2. 0 mg / L+NAA 0. 2 mg / L+KT 0. 1 mg / L; optimal successional medium MS+6-BA 1. 5 mg / L+NAA 0. 2 mg / L+KT 0. 1 mg / L; optimal rooting medium 1 / 2MS+6-BA 0. 5 mg / L+ NAA 0. 3 mg / L+IBA 0. 2 mg / L. This study preliminarily constructed the tissue culture and rapid propa- gation technology process of ‘Wuyuanhuangju’, providing theoretical basis and technical support for the large-scale propagation of ‘Wuyuanhuangju’ seedlings.

‘ Wuyuanhuangju’; medicinal and tea chrysanthemum; the explant; tissue culture; rapid propagation