Journal of Hunan Ecological Science

【Objective】This study aimed to investigate the short-term regulatory effects of Mycobacte- rium phlei preparation on the cecal microbiota of weaned piglets through an in vitro static culture experi- ment, analyzing the impact of M. phlei on the intestinal microbial community. 【Method】Cecal contents from weaned piglets were diluted with physiological saline (1 ∶10) and used as the inoculum, which was inoculated into the medium at a 10% volume. The treatment group was supplemented with 10 μg / mL of M. phlei powder, while an equal volume of sterile quartz sand served as the control group. Both the con- trol and treatment groups were incubated in a 37 ℃carbon dioxide incubator for 4 h. After incubation, samples were centrifuged at 12 000 r/ min for 10 min, and the microbial composition was determined u- sing 16S RNA technology. 【Result】The experimental results showed that the cecal microbiota of weaned piglets was primarily composed of the phyla Firmicutes, Bacteroidetes, and Proteobacteria. Among the top ten genera in terms of microbial abundance were Alloprevotella, Prevotella, Lactobacillus, and Oribac- terium. Compared to the control group, the treatment group exhibited a significant reduction in the num- ber of bacteria such as Oribacterium, Clostridium sensu stricto, and Escherichia coli. Conversely, there was a significant increase in the number of bacteria such as Lactobacillus reuteri, Lactobacillus salivarius, Pediococcus pentosaceus, and Weissella. 【Conclusion】In summary, the M. phlei preparation effectively modulated the abundance of intestinal microbiota in weaned piglets within a short period, demonstrating its positive impact on the intestinal microbial community. This finding provides strong experimental evi- dence for the application of M. phlei in animal husbandry and lays a foundation for further investigation into its mechanism of action and potential applications.

Mycobacterium phlei dry powder; cecum microorganism; gut microorganism; culturein a short time