卷丹与7个百合杂交亲和性的初步研究

Preliminary Study on the Hybridization Compatibility between Lilium lancifolium Thunb. and Seven Lily Species

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DOI 10.3969/j.issn.2095-7300.2024.04.008
刊名
Journal of Hunan Ecological Science
年,卷(期) 2024, 11(4)
作者
作者单位

湖南农业大学园艺学院,湖南长沙;
汨罗市农业农村局,湖南汨罗

摘要
【目的】本研究旨在探究卷丹与不同百合种及品种间的杂交亲和性,以期培育出优良性状的卷丹杂交后代。【方法】将卷丹与7个百合种及品种进行远缘杂交,首先采用液体培养基悬滴法测定父本百合的花粉生活力,杂交后统计杂交组合的果实膨大率,并对获得的杂交蒴果进行胚拯救以获得杂种苗。【结果】实验结果表明,7种百合花粉在9号萌发液中(150g/L蔗糖,200mg/L硼酸,75mg/L氯化钙)普遍萌发较好,其中龙牙百合、‘StrawberryE-vent’的花粉萌发率高于其他父本品种,花粉活力较好。卷丹与亚洲百合杂种系品种远缘杂交的果实膨大率较高,其中与‘StrawberryEvent’杂交的果实膨大率最高,为14.7%,并且只有此杂交组合成功获得了2株杂交后代。【结论】本研究表明卷丹与亚洲百合杂种系杂交亲和性更好,为今后卷丹的远缘杂交育种提供了技术支持。
Abstract
【Objective】The purpose of this study is to explore the hybridization compatibility between the Lilium lancifolium and different lily species and varieties in order to produce the hybrid offspring with excellent traits. 【Method】Lilium lancifolium Thunb. was remotely bred with 7 lily species and varieties. First, the pollen vitality of the paternal lily was measured by the pollen liquid medium method, and the fruit expansion rate of the hybrid combination was counted after crossing, and the hybrid capsule was ob- tained by embryo rescue to obtain hybrid seedlings. 【Result】The results showed that 7 kinds of lily pow- der germinated well in No. 9 germination solution (150 g / L sucrose, 200 mg / L boric acid, 75 mg / L cal- cium chloride), among which the pollen germination rate of Lilium brownii var. viridulum Baker and ‘Strawberry Event’ was higher than that of other parent varieties, and the pollen vitality was better. The hybrid fruit expansion rate of distance hybridization between Lilium lancifolium Thunb. and Asiatic hy- brids was higher, among which the hybridization with ‘Strawberry Event’ had the highest rate of fruit en- largement with 14. 7% , and only this combination successfully obtained two hybrid offspring. 【Conclu- sion】This study shows that the hybridization compatibility between Lilium lancifolium Thunb. and Asiatic lily hybrid line is better, which provides technical support for distant hybrid breeding in the future.
关键词
观赏园艺;卷丹;远缘杂交;杂交亲和性;胚拯救
KeyWord
ornamental horticulture; Lilium lancifolium Thunb. ; distal hybridization; hybridization compatibility; embryo rescue
基金项目
页码 64-70
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陈淼,黄晖,何晶,李玉帆*. 卷丹与7个百合杂交亲和性的初步研究 [J]. 湖南生态科学学报. 2024; 11; (4). 64 - 70.

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