Journal of Hunan Ecological Science

【Objective】It is to expand the population of the hybrid strain LB-2 of Lilium brownii var. viridulum Baker× ‘ Black Stone’ and establish and optimize the in vitro regeneration system of Lilium brownii var. viridulum Baker hybrid offspring. 【Method】This study investigated the effects of different hormone types and concentrations on the induction of adventitious buds and root enlargement of lily scales through orthogonal experimental design. 【Result】The average number of buds and the induction rate of adventitious buds of lily scales were highest in the medium formula of MS medium supplemented with 1. 0 mg / L NAA, 1. 5 mg / L 6-BA, 1. 0 mg / L KT, 30 g / L sucrose, 7. 5 g / L agar, with values of 2. 33 buds and 93. 33% , respectively; from the swelling ratio of the bulbs and the growth status of the root sys- tem, it was found that the lily bulbs had the best swelling ratio of the bulbs and rooting effects in the me- dium formula of MS medium supplemented with 0. 2 mg / L NAA, 0. 5 g / L AC, 80 g / L sucrose, 7. 5 g / L agar. The bulb swelling ratio was 2. 4, and the root system was thick and abundant. 【Conclusion】This study optimized a tissue culture in vitro regeneration system for the entire process from scale induction into buds to small bulb growth, enlargement, and rooting through hormone concentration combinations and optimized hormone configurations. This in vitro regeneration system can provide technical support for the future tissue culture breeding and promotion application of hybrid offspring of Lilium brownii var. viri- dulum Baker.

phytohormone; Lilium brownii var. viridulum Baker; hybrid progeny; proliferation and expansion; bulb enlargement