Sustainable Modulation of Rumen Fermentation and Greenhouse Gas Emissions Using Radish Seeds as a Natural Feed Additive-Journal of Sustainability Research-CSCIED科技核心评价数据库-手机版

Sustainable Modulation of Rumen Fermentation and Greenhouse Gas Emissions Using Radish Seeds as a Natural Feed Additive

Sustainable Modulation of Rumen Fermentation and Greenhouse Gas Emissions Using Radish Seeds as a Natural Feed Additive

ES评分 0 浏览量：373 下载量：0

全文阅读下载引用收藏

DOI	10.20900/jsr20250035
刊名	Journal of Sustainability Research JSR
年，卷(期)	2025, 7(2)
作者	Ahmed E. Kholif,Gouda A. Gouda,Tarek A. Morsy,Hossam H. Azzaz,Md Atikur Rahman,Einar Vargas-Bello-Pérez*,
作者单位	Department of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA ; Dairy Science Department, National Research Centre, 33 Bohouth St. Dokki, Giza 12622, Egyp ; Department of Agriculture, Nutrition and Food Systems, University of New Hampshire, Durham, NH 03824, USA ; Facultad de Zootecnia y Ecología, Universidad Autónoma de Chihuahua, Periférico R. Aldama Km 1, Chihuahua 31031, México ;
Abstract	This study investigated the potential of radish seeds as a sustainable feed additive to enhance nutrient utilization and mitigate greenhouse gas emissions in ruminant systems. Five dietary treatments were formulated by supplementing radish seeds at 0, 0.5, 1.0, 1.5, and 2.0% of dietary dry matter (DM) into a basal diet consisting of concentrate feed mixture, berseem hay, and rice straw (5:4:1 ratio). Key parameters evaluated included in vitro nutrient degradability, ruminal fermentation kinetics, gas production (GP), and emissions of methane (CH4) and carbon dioxide. Gas chromatography analysis identified eucalyptol, α-pinene, and β-pinene as the predominant bioactive compounds in radish seeds. Inclusion of radish seeds led to significant linear (p < 0.001) and quadratic (p < 0.001) increases in asymptotic GP, with the highest values observed at 1.5% and 2.0% inclusion levels. Although CH4 production increased with seed supplementation, its rate exhibited a quadratic trend (p = 0.03), reaching a minimum at 1% inclusion. Radish seeds also linearly improved (p < 0.001) the degradability of DM, neutral detergent fiber, and acid detergent fiber. While total short-chain fatty acids (SCFA) and acetate concentrations increased (p = 0.004), propionate and butyrate remained unaffected. Additionally, ammonia-N concentration, microbial protein synthesis, and partitioning factor showed both linear and quadratic responses (p < 0.05 and p < 0.019, respectively). These findings highlight the potential of radish seeds to improve fermentation efficiency and reduce the environmental footprint of ruminant production systems, contributing to more sustainable livestock feeding strategies.
KeyWord	radish seeds; degradability; in vitro fermentation; methane; phytogenics; sustainability
基金项目
页码	-

参考文献
相关文献

1.Bačėninaitė D, Džermeikaitė K, Antanaitis R. Global warming and dairy cattle: How to control and reduce methane emission. Animals. 2022;12:2687. doi: 10.3390/ani12192687.

2.Kholif AE. A review of effect of saponins on ruminal fermentation, health and performance of ruminants. Vet Sci. 2023;10:450. doi: 10.3390/vetsci10070450.

3.Ike KA, Adelusi OO, Alabi JO, Olagunju LK, Wuaku M, Anotaenwere CC, et al. Effects of different essential oil blends and fumaric acid on in vitro fermentation, greenhouse gases, nutrient degradability, and total and molar proportions of volatile fatty acid production in a total mixed ration for dairy cattle. Agriculture. 2024;14:876. doi: 10.3390/agriculture14060876.

4.Wang J, Deng L, Chen M, Che Y, Li L, Zhu L, et al. Phytogenic feed additives as natural antibiotic alternatives in animal health and production: A review of the literature of the last decade. Animal Nutrition. 2024;17:244-64.

5.Orffer Z, van Zyl JHC, Semwogerere F, Mapiye C, Strydom PE. Substituting monensin in lamb finisher diets with a Citral and Linalool blend on meat physicochemical properties, shelf-display stability and fatty acid composition. Anim Feed Sci Technol. 2025;321:116256. doi: 10.1016/j.anifeedsci.2025.116256.

6.Kholif AE, Gouda GA, Fahmy M, Morsy TA, Abdelsattar MM, Vargas‐Bello‐Pérez E. Fennel seeds dietary inclusion as a sustainable approach to reduce methane production and improve nutrient utilization and ruminal fermentation. An Sci J. 2024;95:13910. doi: 10.1111/asj.13910.

7.Morsy TA, Kholif AE, Adegbeye MJ, Olafadehan OA, Gouda GA, Fahmy M, et al. Lupin seed supplementation as a functional feed additive: In Vitro ruminal gas, methane and carbon dioxide production, fermentation kinetics, and nutrient degradability. Animals. 2024;14:2119. doi: 10.3390/ani14142119.

8.Kholif AE. The impact of varying levels of Laurus nobilis leaves as a sustainable feed additive on ruminal fermentation: in vitro gas production, methane and carbon dioxide emissions, and ruminal degradability of a conventional diet for ruminants. Fermentation. 2024;10:387. doi: 10.3390/fermentation10080387.

9.Keim JP, Gandarillas M, Benavides D, Cabanilla J, Pulido RG, Balocchi OA, et al. Nutrient concentrations and profile of non-structural carbohydrates vary among different Brassica forages. Anim Prod Sci. 2020;60:1503-13.

10.Aly AA, Maraei RW, Sharafeldin RG, Safwat G. Yield traits of red radish seeds obtained from plants produced from γ-irradiated seeds and their oil characteristics. Gesunde Pflanzen. 2023;75:2089-99. doi: 10.1007/s10343-023-00859-8.

11.Mbambalala L, Rani ZT, Mpanza TDE, Mthana MS, Ncisana L, Mkhize NR. Fodder radish as a potential alternative feed source for livestock in South Africa. Agriculture. 2023;13:1625. doi: 10.3390/agriculture13081625.

12.Galanty A, Grudzińska M, Paździora W, Służały P, Paśko P. Do Brassica vegetables affect thyroid function?—A comprehensive systematic review. Int J Mol Sci. 2024;25(7):3988.

13.Jeon S, Sohn K-N, Seo S. Evaluation of feed value of a by-product of pickled radish for ruminants: Analyses of nutrient composition, storage stability, and in vitro ruminal fermentation. J Anim Sci Technol. 2016;58:34. doi: 10.1186/s40781-016-0117-1.

14.Goering HK, Van Soest PJ. Forage fiber analyses. (Apparatus, Reagents, Procedures and Some Applications) Agriculture Handbook No. 379. Washington (DC, US): Agricultural Research Service, USA; 1970.

15.AOAC. Official Methods of Analysis of AOAC International. 16th ed. Washington (DC, US): AOAC International; 1997.

16.Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci. 1991;74:3583-97.

17.France J, Dijkstra J, Dhanoa MS, Lopez S, Bannink A. Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro: Derivation of models and other mathematical considerations. Br J Nutr. 2000;83:143-50.

18.Blümmel M, Steingaβ H, Becker K. The relationship between in vitro gas production, in vitro microbial biomass yield and 15N incorporation and its implications for the prediction of voluntary feed intake of roughages. Br J Nutr. 1997;77:911-21. doi: 10.1079/BJN19970089.

19.Menke KH, Raab L, Salewski A, Steingass H, Fritz D, Schneider W. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. J Agric Sci. 1979;93:217-22. doi: 10.1017/S0021859600086305.

20.Hereshko H, Voloshchuk I, Voloshchuk O, Hlyva V, Bilonizhka K. Oilseed radish—valuable crop of a wide range use. Foothill and mountain agriculture and stockbreeding. 2021;70:8-17. doi: 10.32636/01308521.2021-(70)-2-1.

21.Gamba M, Asllanaj E, Raguindin PF, Glisic M, Franco OH, Minder B, et al. Nutritional and phytochemical characterization of radish (Raphanus sativus): A systematic review. Trends Food Sci Technol. 2021;113:205-18. doi: 10.1016/j.tifs.2021.04.045.

22.Baranauskiene R, Dambrauskiene E, Venskutonis PR, Viskelis P. Influence of harvesting time on the yield and chemical composition of sage (Salvia officinalis L.). In: 6th Baltic Conference on Food Science and Technology: Innovations for Food Science and Production, FOODBALT-2011—Conference Proceedings; 2011. p. 104-9.

23.Park CH, Park S-Y, Park YJ, Kim JK, Park SU. Metabolite profiling and comparative analysis of secondary metabolites in Chinese cabbage, radish, and hybrid xBrassicoraphanus. J Agric Food Chem. 2020;68:13711-9. doi: 10.1021/acs.jafc.0c04897.

24.Bellani LM, Salvini L, Dell’Aquila A, Scialabba A. Reactive oxygen species release, vitamin E, fatty acid and phytosterol contents of artificially aged radish (Raphanus sativus L.) seeds during germination. Acta Physiol Plant. 2012;34:1789-99. doi: 10.1007/s11738-012-0976-0.

25.Benchaar C, Calsamiglia S, Chaves AV, Fraser GR, Colombatto D, McAllister TA, et al. A review of plant-derived essential oils in ruminant nutrition and production. Anim Feed Sci Technol. 2008;145:209-28. doi: 10.1016/j.anifeedsci.2007.04.014.

26.Woodward SL, Waghorn GC, Ulyatt MJ, Lassey KR. Early indications that feeding Lotus will reduce methane emissions from ruminants. Proc N Z Soc Anim Prod. 2001;61:23-6. Available from: http://www.nzsap.org/proceedings/2001/early-indications-feeding-lotus-will-reduce-methane-emissions-ruminants. Accessed on 16 Mar 2024.

27.de Sousa DP, Damasceno ROS, Amorati R, Elshabrawy HA, de Castro RD, Bezerra DP, et al. Essential oils: Chemistry and pharmacological activities. Biomolecules. 2023;13:1144. doi: 10.3390/biom13071144.

28.Calsamiglia S, Busquet M, Cardozo PW, Castillejos L, Ferret A. Invited review: Essential oils as modifiers of rumen microbial fermentation. J Dairy Sci. 2007;90:2580-95. doi: 10.3168/jds.2006-644.

29.Macheboeuf D, Morgavi DP, Papon Y, Mousset J-L, Arturo-Schaan M. Dose–response effects of essential oils on in vitro fermentation activity of the rumen microbial population. Anim Feed Sci Technol. 2008;145:335-50. doi: 10.1016/j.anifeedsci.2007.05.044.

30.Getachew G, Robinson PH, DePeters EJ, Taylor SJ. Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds. Anim Feed Sci Technol. 2004;111:57-71. doi: 10.1016/S0377-8401(03)00217-7.

31.Blümmel M, Makkar HPS, Becker K. In Vitro gas production: A technique revisited. J Anim Physiol Anim Nutr (Berl). 1997;77:24-34. doi: 10.1111/j.1439-0396.1997.tb00734.x.

32.Kholif AE, Gouda GA, Morsy TA, Matloup OH, Fahmy M, Gomaa AS, et al. Dietary date palm leaves ensiled with fibrolytic enzymes decreased methane production, and improved feed degradability and fermentation kinetics in a ruminal in vitro system. Waste Biomass Valor. 2022;13:3475-88. doi: 10.1007/s12649-022-01752-7.

33.Kholif AE, Gouda GA, Morsy TA, Matloup OH, Sallam SM, Patra AK. Associative effects between Chlorella vulgaris microalgae and Moringa oleifera leaf silage used at different levels decreased in vitro ruminal greenhouse gas production and altered ruminal fermentation. Environ Sci Pollut Res. 2023;30:6001-20. doi: 10.1007/s11356-022-22559-y.

34.Kholif AE, Olafadehan OA. Essential oils and phytogenic feed additives in ruminant diet: Chemistry, ruminal microbiota and fermentation, feed utilization and productive performance. Phytochem Rev. 2021;20:1087-108. doi: 10.1007/s11101-021-09739-3.

35.Broudiscou LP, Papon Y, Broudiscou AF. Effects of dry plant extracts on fermentation and methanogenesis in continuous culture of rumen microbes. Anim Feed Sci Technol. 2000;87:263-77. doi: 10.1016/S0377-8401(00)00193-0.

36.Sallam SMA, Bueno ICS, Brigide P, Godoy PB, Vitti DMS, Abdalla AL. Production in Efficacy of eucalyptus oil on in vitro ruminal fermentation and methane production. In: Papachristou TG, Parissi ZM, Ben Salem H, Morand-Fehr P, editors. Options Méditerranéennes: Série A. Séminaires Méditerranéens. Zaragoza (Spain): CIHEAM/FAO/NAGREF; 2009. p. 267-72.

37.Bryszak M, Szumacher-Strabel M, Huang H, Pawlak P, Lechniak D, Kołodziejski P, et al. Lupinus angustifolius seed meal supplemented to dairy cow diet improves fatty acid composition in milk and mitigates methane production. Anim Feed Sci Technol. 2020;267:114590.

38.Belle AJ, Lansing S, Mulbry W, Weil RR. Methane and hydrogen sulfide production during co-digestion of forage radish and dairy manure. Biomass Bioenergy. 2015;80:44-51. doi: 10.1016/j.biombioe.2015.04.029.

39.Haque MN. Dietary manipulation: A sustainable way to mitigate methane emissions from ruminants. J Anim Sci Technol. 2018;60:15.

40.Laporte-Uribe JA. The role of dissolved carbon dioxide in both the decline in rumen pH and nutritional diseases in ruminants. Anim Feed Sci Technol. 2016;219:268-79. doi: 10.1016/j.anifeedsci.2016.06.026.

41.Kumar S, Dagar SS, Sirohi SK, Upadhyay RC, Puniya AK. Microbial profiles, in vitro gas production and dry matter digestibility based on various ratios of roughage to concentrate. Ann Microbiol. 2013;63:541-5. doi: 10.1007/s13213-012-0501-0.

42.Maheri-Sis N, Chamani M, Sadeghi A-A, Mirza-Aghazadeh A, Aghajanzadeh-Golshani A. Nutritional evaluation of kabuli and desi type chickpeas (Cicer arietinum L.) for ruminants using in vitro gas production technique. Afr J Biotechnol. 2008;7:2946-51.

43.Azar M, Doust-Nober RS, Sis NM, Shahryar H, Asadi Y. Effects of Zataria multiflora extract as rumen modifiers using in vitro gas production Technique. Curr Res J Biol Sci. 2012;4:350-54.

44.Fellner V. Rumen Microbes and Nutrient Management. Raleigh (NC, USA): North Carolina State University; 2004.

45.Almeida M, Garcia-Santos S, Nunes A, Rito S, Azevedo J, Guedes C, et al. Introducing mediterranean lupins in Lambs’ diets: Effects on growth and digestibility. Animals. 2021;11:942. doi: 10.3390/ani11040942.

46.Gomaa AS, Kholif AE, Kholif AM, Salama R, El-Alamy HA, Olafadehan OA. Sunflower oil and Nannochloropsis oculata microalgae as sources of unsaturated fatty acids for mitigation of methane production and enhancing diets’ nutritive value. J Agric Food Chem. 2018;66:1751-9. doi: 10.1021/acs.jafc.8b00324.

47.Urrutia NL, Harvatine KJ. Acetate dose-dependently stimulates milk fat synthesis in lactating dairy cows. J Nutr. 2017;147:763-9. doi: 10.3945/jn.116.245001.

48.Wang L, Zhang G, Li Y, Zhang Y. Effects of high forage/concentrate diet on volatile fatty acid production and the microorganisms involved in VFA production in cow rumen. Animals. 2020;10(2):223.

49.Bauman DE, Griinari JM. Regulation and nutritional manipulation of milk fat: Low-fat milk syndrome. Livest Prod Sci. 2001;70:15-29.

50.Allen MS, Piantoni P. Metabolic control of feed intake. Implications for metabolic disease of fresh cows. Vet Clin North Am—Food Anim Pract. 2013;29:279-97.

51.Kholif AE, Hassan AA, El Ashry GM, Bakr MH, El-Zaiat HM, Olafadehan OA, et al. Phytogenic feed additives mixture enhances the lactational performance, feed utilization and ruminal fermentation of Friesian cows. Anim Biotechnol. 2021;32:708-18. doi: 10.1080/10495398.2020.1746322.

52.Salem AZM, Kholif AE, Elghandour MMY, Hernandez SR, Domínguez‐Vara IA, Mellado M. Effect of increasing levels of seven tree species extracts added to a high concentrate diet on in vitro rumen gas output. An Sci J. 2014;85:853-60. doi: 10.1111/asj.12218.

53.Garcia-Santos S, Almeida M, Closson M, Guedes CM, Barros A, Ferreira LM, et al. Effect of total replacement of the soya bean meal by lupine seeds (L. albus and L. luteus) on performance and digestion characteristics of growing rabbits. Anim Feed Sci Technol. 2021;278:114996. doi: 10.1016/j.anifeedsci.2021.114996.

54.Lee SS, Kim DH, Paradhipta DHV, Lee HJ, Yoon H, Joo YH, et al. Effects of wormwood (Artemisia montana) essential oils on digestibility, fermentation indices, and microbial diversity in the rumen. Microorganisms. 2020;8:1605. doi: 10.3390/MICROORGANISMS8101605.

55.Arjun S, Neha P, Mohith Sai SR, Ravi L. Microbial symbionts in ruminants. In: Dharumadurai D, editor. Microbial symbionts: Functions and molecular interactions on host. Cambridge (MA): Academic Press; 2023. p. 493-509. doi: 10.1016/B978-0-323-99334-0.00011-6.

56.Nagaraja TG, Titgemeyer EC. Ruminal acidosis in beef cattle: The current microbiological and nutritional outlook. J Dairy Sci. 2007;90S:E17-38. doi: 10.3168/jds.2006-478.

57.Um KH, Shin JS, Son GH, Park BK. Effect of lupin supplementation on the growth, carcass, and meat characteristics of late-fattening Hanwoo steers. Animals. 2024;14:324. doi: 10.3390/ani14020324.

58.Kumar K, Dey A, Rose MK, Dahiya SS. Modulating feed digestion and methane production by eucalyptus (Eucalyptus citriodora) leaves essential oils in water buffalo (Bubalus bubalis). Buffalo Bulletin. 2022;41:41-7. doi: 10.56825/bufbu.2022.4113155.

59.Firkins JL, Yu Z, Morrison M. Ruminal nitrogen metabolism: Perspectives for integration of microbiology and nutrition for dairy. J Dairy Sci. 2007;90S:E1-16. doi: 10.3168/jds.2006-518.

60.Thirumalesh T, Krishnamoorthy U. Rumen microbial biomass synthesis and its importance in ruminant production. Int J Livest Res. 2013;3:5. doi: 10.5455/ijlr.20130502081346.

61.Rodríguez R, Sosa A, Rodríguez Y. Microbial protein synthesis in rumen and its importance to ruminants. Cuban J Agr Sci. 2007;41:287-94.

62.Toro M-T, Fustos-Toribio R, Ortiz J, Becerra J, Zapata N, López-Belchí MD. Antioxidant responses and phytochemical accumulation in Raphanus species sprouts through elicitors and predictive models under high temperature stress. Antioxidants. 2024;13:333. doi: 10.3390/antiox13030333.

63.NRC. Nutrient Requirements of Dairy Cattle. Washington (DC, US): National Academies Press; 2001. doi: 10.17226/9825.

64.Lu Z, Xu Z, Shen Z, Tian Y, Shen H. Dietary energy level promotes rumen microbial protein synthesis by improving the energy productivity of the ruminal microbiome. Front Microbiol. 2019;10:847. doi: 10.3389/fmicb.2019.00847.

引用本文

Ahmed E. Kholif,Gouda A. Gouda,Tarek A. Morsy,Hossam H. Azzaz,Md Atikur Rahman,Einar Vargas-Bello-Pérez*. Sustainable Modulation of Rumen Fermentation and Greenhouse Gas Emissions Using Radish Seeds as a Natural Feed Additive, Journal of Sustainability Research. 2025; 7; (2). https://doi.org/10.20900/jsr20250035.

文献评论

相关学者

相关机构