| Abstract |
OBJECTIVE: To investigate the correlation between peripheral blood BDNF expression levels and their cognitive impairment in patients with schizophrenia.METHODS: 54 schizophrenia patients meeting the criteria and 55 healthy controls were selected. The clinical symptoms of schizophrenia patients were assessed using the PANSS scale, and the cognitive function of the subjects was assessed using the MCCB cognitive function test tool. The relative expression of peripheral blood microRNA124-3p and BDNF mRNA were measured by reverse transcription real-time quantitative fluorescence polymerase chain reaction (RT-qPCR), and the concentration of brain-derived neurotrophic factor (BDNF) in peripheral blood was measured by enzyme-linked immunoassay (ELISA).RESULTS: 1. Compared with the control group, the patient group showed elevated microRNA124-3p expression (P<0.001), decreased BDNFmRNA and BDNF expression (P<0.001), and significantly decreased in four areas of information processing speed, word learning, reasoning and problem solving, and visual learning in MCCB (P<0.001). 2. The total PANSS score, positive symptom score, and negative symptom score were used as dependent variables, respectively, and microRNA124-3p relative expression, BDNFmRNA relative expression, and BDNF concentration were used as independent variables . Using multiple linear regression analysis, the results showed that BDNFmRNA relative expression in the patient group was a positive influence on PANSS total score (P<0.05), microRNA124-3p relative expression was a negative influence factor on positive symptom score (P<0.05), and no other correlations were seen. 3. Multiple linear regression analysis was performed with SOP-T score, VEL-T score, RPS-T score and VIL-T score as dependent variables, and PANSS total score, positive symptom score, negative symptom score, microRNA124-3p relative expression, BDNF mRNA relative expression and BDNF concentration as independent variables, respectively, and the results showed that in the patient group negative symptom score was a positive influence of VIL-T score (P<0.05), in the patient microRNA124-3p relative expression was a negative influence of RPS-T score (P<0.05), in the patient BDNF protein factor concentration was a positive influence of RPS-T score (P<0.05) , in the control group BDNF protein factor concentration was a positive influence of RPS-T score (P< 0.05), and no other correlations were seen.CONCLUSION: Peripheral blood BDNF expression in schizophrenia patients was negatively correlated with the severity of their cognitive impairment.
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