International Journal of Medicine and Data

Objective To develop biochemical quality control substance for cerebrospinal fluid and evaluate its stability. Methods Different concentration cerebrospinal fluid(CSF) quality control materials(self-maded CSF) were prepared and stored at 4℃-20℃ and 25 ℃ for 28 days, then using automatic biochemical analyzer to determine the concentrations of chlorideion (CL), glucose (GLU), cerebrospinal fluid protein (CSFP), lactic acid Dehydrogenase (LDH) and adenosine deaminase (ADA) ,Coefficient of variation (CV%) was calculated to evaluate its stability and accuracy was evaluated by recovery test. The stability of self-maded CSF, clinical collected CSF and commercial CSF stored at 4℃ for 60 days was evaluated. Results The CV% of CL\GLU\LDH\ADA was the smallest in self-maded CSF stored at 4°C for 28 days;There were significant differences between the normal and B concentrations in all items of self-maded CSF(P<0.05).The recovery rates of normal concentrations quality control products were CL: 88% , GLU: 89% , CSFP: 104% , LDH: 98% , ADA: 107%;after stored at 4°C for 60 days, the value of CV% of CL\GLU\ADA in the self-maded normal concentration CSF was significantly lower than that of clinical collection CSF and commercial CSF. The change of CSFP was similar to that of clinical collection CSF and significantly smaller than that of commercial CSF, but the concentration of LDH was significantly higher than that of clinical collection CSF. Conclusion The self-maded CSF has good stability when stored at 4℃ for 28 days. The normal concentration and B concentration of the self-maded CSF may meet requirements of high-value and low-value quality control products. Most of the items in normal concentration self-maded CSF have a good quality. The normal concentration of self-maded CSF has better stability at 4 ℃ for 60 days,the stability of all items in the self-maded CSF is better than that of commercial CSF and clinical CSF. The self-maded CSF can meet the routine use in clinical laboratories.

Cerebrospinal fluid; Quality control substance; Stability; Evaluation