International Journal of Clinical Research

Objective To investigate the biological behaviors of SW480 cells such as proliferation, invasion and migration affected by miRNA-140-5p through targeted regulation of FoxM1. Methods The dual luciferase reporter gene system was used to identify the targeted matching relationship between miR-140-5p and FoxM1. CCK8 was used to detect the effect of FoxM1 gene expression on cell proliferation, and the regulatory effect of miR-140-5p on FoxM1 expression. The effect of FoxM1 on cell growth was detected by cell colony formation assay, the expression of FoxM1 protein was detected by Western blotting, and the invasion ability of FoxM1 on SW480 cells was observed by Transwell assay. Results Dual luciferase reporter gene system identification showed that miR-140-5p effectively regulated the expression of FoxM1 after transcription. CCK8 detection showed that high expression of FoxM1 gene could promote the proliferation of cancer cells. The results of cell colony formation assay showed that high expression of FoxM1 gene could increase the cloning ability of SW480 cells. The results of cell scratch assay showed that FoxM1 gene played an important role in the migration ability of colorectal cancer SW480 cells, and miR-140-5p inhibited the migration ability of cells. Conclusion miR-140-5p can target and regulate FoxM1. Knockdown of FoxM1 gene can inhibit the malignant process of SW480 cells. miR-140-5p may participate in the biological behaviors of SW480 cell proliferation, invasion and migration by targeting and regulating FoxM1.

microRNA; Colorectal tumor; Proliferation; Invasion