Scientific Development Research

With the escalating public focus on food safety, the expedited detection of foodborne pathogenic bacteria has emerged as a pivotal strategy for the prevention and management of foodborne diseases. The PCR detection method, extensively utilized in molecular biology and medical diagnostics, is also critically important for the identification of foodborne pathogens. This scholarly article presents a comprehensive overview within this context, analyzing pertinent concepts and synthesizing research on foodborne pathogen detection technologies, PCR detection methodologies, PCR detection standards, and the rapid identification of foodborne pathogens via duplex PCR. It is intended to serve as a technical reference for professionals engaged in food safety testing.

Duplex Polymerase Chain Reaction (PCR) methodology; Foodborne pathogens; Expedited detection protocols; Scholarly review