Journal of Agriculture and Food Science

As people pay more and more attention to food safety issues, the technology suitable for rapid food safety detection in the field has attracted more and more attention from Chinese scholars. Recombinase Polymerase Amplification (RPA) is a newly established isothermal nucleic acid amplification technique. Compared with the traditional nucleic acid detection technology, RPA technology has the characteristics of fast detection speed, low cost, little dependence on equipment, etc., which is very suitable for carrying out on-site food safety detection work in places with poor conditions or insufficient resources. This paper introduces the principle of RPA amplification and the principle of the most common RPA endpoint detection technology, lateral flow chromatography (LED). The application of RPA technology in detection of foodborne pathogenic microorganisms, identification of species components, identification of genetically modified food and identification of allergens in food was reviewed. The advantages and challenges of the application of RPA technology in the field of food safety inspection are summarized and analyzed. The future development prospect of RPA technology is also prospected.

Recombinase polymerase amplification technique (RPA); Isothermal nucleic acid amplification technique; Food safety testing; Foodborne pathogenic microorganisms; Species composition identification; Genetically modified food; Allergen component