Journal of Modern Biotechnology Research

Objectives: Study the effects of high expression of Mu opioid receptors (MOR) on the proliferation and migration of vascular endothelial cells. Methods: Collect intracranial stenotic arteries and collateral arteries from intracranial arterial stenosis (ICS) rat model, extract proteins and detect the expression of MOR through Western blotting method; use plasmid to highly express MOR, and conduct microtubule formation experiments. Colony formation assay and Transwell assay were used to observe the proliferation and migration of vascular endothelial cells. Results: Western blotting showed that MOR expression in the collateral branches of ICS rats was significantly increased compared with the normal control group. The microtubule formation experiment showed that compared with the control group, the MOR high expression group significantly promoted the formation of microtubules in vascular endothelial cells. The vascular endothelial cell colony formation experiment showed that compared with the control group, the MOR high expression group significantly promoted the proliferation of vascular endothelial cells. Transwell experiments confirmed that compared with the control group, the MOR high expression group significantly promoted the migration of vascular endothelial cells. Conclusion: MOR is highly expressed in the collateral arteries of ICS rats, and increased MOR expression can significantly promote the proliferation and migration of vascular endothelial cells.

Mu opioid receptors (MOR); vascular endothelial cells; intracranial artery stenosis; collateral circulation