炎症性肠病患者血清胆汁酸谱的液质检测与结果分析-国际临床研究杂志-CSCIED科技核心评价数据库-手机版

炎症性肠病患者血清胆汁酸谱的液质检测与结果分析

Detection and analysis of serum bile acid profiles using liquied chromatography tandem mass spectrometry in patients with inflammatory bowel disease

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DOI	10.12208/j.ijcr.20240333
刊名	国际临床研究杂志 International Journal of Clinical Research
年，卷(期)	2024, 8(8)
作者	刘楚*,董维,张吉,沈凌晓,吴卫甲,
作者单位	杭州凯莱谱医学检验实验室有限公司浙江杭州 ;
摘要	通过液相色谱串联质谱法（LC-MS/MS）方法准确检测炎症性肠病患者血清中的胆汁酸谱水平，探讨血清胆汁酸谱的检测结果在炎症性肠病患者的临床诊断、早期预防及治疗靶点中的应用价值。方法入组2019年9月-2023年11月间来我司进行血清胆汁酸谱检测的412例克罗恩病患者、198例溃疡性结肠炎患者、121例健康体检者，采用LC-MS/MS检测其血清胆汁酸中的15种亚组份,包括：胆酸（CA）、甘氨胆酸（GCA）、牛磺胆酸（TCA）、鹅脱氧胆酸（CDCA）、甘氨鹅脱氧胆酸（GCDCA）、牛磺鹅脱氧胆酸（TCDCA）、脱氧胆酸（DCA）、甘氨脱氧胆酸（GDCA）、牛磺脱氧胆酸（TDCA）、石胆酸（LCA）、甘氨石胆酸（GLCA）、牛磺石胆酸（TLCA）、熊脱氧胆酸（UDCA）、甘氨熊脱氧胆酸（GUDCA）、牛磺熊脱氧胆酸（TUDCA），并进行各组之间结果比较。结果与健康组相比较，溃疡性结肠炎组和克罗恩病组的初级胆汁酸水平要高于健康组，其中游离型的CA和CDCA要明显高于健康组，差异均有统计学意义（P<0.05）；次级胆汁酸大部分要低于健康组，其中DCA、LCA、GDCA、GLCA的差别均有统计学意义（P<0.05）。结论血清胆汁酸谱的LC-MS检测结果可能作为炎症性肠病临床诊断及早期预防、治疗的重要辅助手段。
Abstract	Objective: To explore the change of serum bile acid concentrations using liquid chromatography tandem mass spectrometry (LC-MS/MS) and assess the application of the results in the clinical diagnosis, early prevention and treatment of inflammatory bowel disease. Methods The relevant clinical data was collected from 412 Crohn disease patients 、198 ulcerative colitis patients and 121healthy individuals undergoing serum bile acid testing from September 2019 - November 2023 at Hangzhou CALIBRA Medical Laboratory . Fifteen serum bile acids were detected by liquid chromatography tandem mass spectrometry. Results Compared with the healthy control group, the primary bile acids in the ulcerative colitis group and the Crohns disease group were higher, such as the changed CA and CDCA levels which were statistically significant (P<0.05). The secondary bile acids were mostly lower in the ulcerative colitis group and the Crohns disease group, such as the changed DCA, LCA, GDCA, and GLCA levels which were statistically significant (P<0.05). Conclusion Serum bile acid profiling using LC-MS/MS could possibly be used as an important supplement for the clinical diagnosis, early prevention and treatment of inflammatory bowel disease.
关键词	炎症性肠病；血清胆汁酸谱；液相色谱串联质谱；肠道菌群
KeyWord	Inflammatory bowel disease; Serum bile acid profile; Liquid chromatography tandem mass spectrometry; Gut microbiota
基金项目
页码	174-180

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刘楚*,董维,张吉,沈凌晓,吴卫甲. 炎症性肠病患者血清胆汁酸谱的液质检测与结果分析 [J]. 国际临床研究杂志. 2024; 8; (8). 174 - 180.

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