International Journal of Clinical Research

Objective: To investigate the relationship between the histopathological changes of testis in a mouse cryptorchidism model and the levels of serum UCHL-1 and related sex hormones. Methods Fifty ICR male mice were reared, and the rats were randomly divided into three groups using a random number table: sham operation group, unilateral cryptorchidism group and bilateral cryptorchidism group. In the unilateral group and bilateral group, the corresponding testis was surgically fixed into the abdominal cavity, and the testis tissue in the sham operation group was not treated with special treatment after the abdominal cavity was opened. The bilateral testicular tissues were washed, weighed, and immersed in formaldehyde solution for preservation. Serum hormone levels were measured by enzyme-linked immunosorbent assay (ELISA kit), testicular tissue was stained with HE, observed under a light microscope and scored according to the Johnsen scoring standard. Results There was no significant difference in the body weight of the experimental mice in each group (P<0.05). Testicular weight, testis volume: There were statistically significant differences between the contralateral testis and the affected testis in the unilateral group, and the average testis volume (weight) in the bilateral group and the sham operation group (P<0.05). Serum AMH and INH-B concentrations. Compared with the unilateral group, the bilateral group and the sham operation group, there were statistically significant differences among the three groups (P<0.05). Conclusion Cryptorchidism can cause testicular tissue dysfunction in mice, and it can be considered that serum UCHL-1 is a simple and accurate serum marker, which can well evaluate the function of testicular tissue in cryptorchidism.

cryptorchidism; mice; sex hormones; UCHL-1