International Medical Research Frontier

Objective To establish UPLC-M S/MS method to quickly detect aphrodisiac and weight loss chemicals in tablet candy. Methods The test samples were extracted with methanol, separated by Agela Technologies Venusil MP C18 column (3.0 mm*100 mm, 3 μ m), gradient eluted with 0.1% formic acid in water and 0.1% formic acid acetonitrile as mobile phase; electrospray ion source (ESI), positive ion mode, multiple reaction monitoring (MRM) for detection. Results In this method, 17 components were well separated, with good linear relationship within 2 to 200 ng/mL, correlation coefficient greater than 0.991; the average recovery of low, medium and high addition levels was 81.7%~105.2%, the relative standard deviation was 3.8%~8.7%, and the limit of quantification was 1.35~330 μ g / kg. Conclusion This method has high sensitivity and simple operation, and can be used for quantitative analysis of 17 illegal chemical drug ingredients in tabletcandy.

UPLC-M S/MS; tabletcandy; illegal addition