International Research in Chinese Medicine

Objective To study the effect of Wumen RMK-03TM active components on the cell proliferation and expression of cytokines in LPS-induced nasal mucosa epithelial cells. Methods HNEpC cells were cultured in vitro and the effect of Wumen RMK-03TM active components at different doses (0, 1, 2, 4, 8, 16mg/L) on the proliferation of human nasal mucosa epithelial cells was detected by CCK8 method. The cells were divided into four groups, blank control group (not treated), LPS group (treated with 100mg/L LPS for 24 hours), and LPS + RMK-03TM active components groups (4mg/L or 8mg/L components for 24hours after being treated with 100mg/L LPS for 24 hours). The proliferation of nasal mucosa epithelial cells was detected by CCK8 method. The contents of IL-1β and TNF-α a in the cell supernatant were detected by ELISA method. The expressions of p-IκBα, p-P65 and ACE2 proteins were detected by Western blot. Results The proliferation activity of nasal mucosa epithelial cells increased after RMK-03TM active components treatment, especially the RMK-03TM active components at 416mg/L. Compared with the blank control group, the proliferation activity of LPS group was decreased, the contents of IL-1β, TNF-α and p-IκBα, p-P65, ACE2 protein were increased significantly (P＜0.05). Compared with LPS group, the proliferation activity of LPS + RMK-03TM active components groups was increased, the contents of IL-1β, TNF-α and p-IκBα, p-P65, ACE2 proteins were decreased (P＜0.05). Conclusion Wumen RMK-03TM active components can promote the proliferation of LPS induced nasal mucosa epithelial cells and inhibit the secretion of inflammatory cytokines. The possible mechanism is that RMK-03TM active components can inhibit the expression of the p-IκBα, p-P65 and ACE2 proteins.

Wumen RMK-03TM; HNEpC epithelial cells; LPS; cell injury