| Abstract |
In this study, the egg preservation techniques of Siberian sturgeon were studied. The parameters were specifically set as egg fertilization rate, and the experimental effects of various types, different amounts of preservatives, diluents, and different dilution degrees on egg preservation of experimental fish were mainly studied. Studies have shown that the egg preservation solution of Siberian sturgeon is composed of the following parts: culture solution 60%~70%, nutrient solution 15%~20%, fish ovarian fluid 9%~23% and antibacterial solution 1%~2%. Among them, Glycine 0.5~0.6g/L, glucose 1.0~2.0g/L, HEPES0.8~1.2g/L, sodium dihydrogen phosphate 0.2~0.4g/L, potassium chloride 0.17~0.38g/L, magnesium sulfate 0.2~0.23g/L, sodium chloride 5.0~7.25g/L, calcium chloride 0.11~0.13g/ L L, sodium bicarbonate 2.0~2.5g/L, bovine serum albumin 0.5~1.0g/L, penicillin 300 ~ 350,000 IU/L, streptomycin sulfate 0.2~0.4g/L. The optimal egg storage is 1 ml /200 eggs. The optimal egg preservation solution by volume consists of the following components: culture solution 65%, nutrient solution 17.5%, fish ovarian solution 16%, and antibacterial solution 1.5%. The results of this study can lay a solid foundation for the future conservation of the germplasm resources of rare acipenser in the Yangtze River.
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