T、B淋巴细胞亚类的方法学建立及性能评价
Methodological establishment and performance evaluation of T and B Lymphocyte subclasses
ES评分 0
| DOI |
10.12208/j.imrf.20210008 |
| 刊名 |
International Medical Research Frontier
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| 年，卷(期) |
2021, 5(1) |
| 作者 |
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| 作者单位 |
河北省邯郸市第一医院检验科 河北邯郸 ;
北京大学第一医院 北京 ;
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| 摘要 |
T、B淋巴细胞亚类的方法学建立及性能评价。方法 通过查阅文献确立T、B淋巴细胞亚类的抗体组合，沿用溶血洗涤的标本处理方法。基于中华人民共和国卫生部《流式细胞术检测外周血淋巴细胞亚群指南》推荐的方法，建立适用于流式细胞仪性能评价的测试方案，包括精密度、试剂稳定性、抗体量对结果的影响等。结果 T、B淋巴细胞亚类的方法学初步建立，性能评价初步确立。结论 上述性能指标测试方法可靠，能够对T、B淋巴细胞亚类检测性能进行全面评价。该方法对于T、B淋巴细胞亚类检测性能验证有一定的指导意义。
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| Abstract |
Objective To establish the methodology and evaluate the performance of T and B lymphocyte subsets. Methods: the antibody combination of T and B lymphocyte subclasses was established by consulting literature, and the method of hemolytic washing was used. Based on the method recommended by the State Food and drug administration, a test scheme suitable for the performance evaluation of FCM was established, including the precision, the stability of reagents ,the influence about the amount of antibody. Results: the methods of T and B lymphocyte subsets were established preliminarily, and the performance evaluation met the requirements. Conclusion: the above methods are reliable and can be used to evaluate the detection performance of T and B lymphocyte subsets. This method has certain guiding significance for the verification of the detection performance of T and B lymphocyte subsets.
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| 关键词 |
流式细胞仪；方法学建立；性能评价
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| KeyWord |
Flow Cytometry; Methodology Establishment; Performance Evaluation
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| 基金项目 |
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| 页码 |
19-22 |
张立霞*,屈晨雪.
T、B淋巴细胞亚类的方法学建立及性能评价 [J].
国际医药研究前沿.
2021; 5; (1).
19 - 22.