International Journal of Clinical Research

Objective To evaluate the performance of electrochemiluminescence and immunofluorescence in the detection of procalcitonin. Methods: 255 serum samples were collected and detected by electrochemiluminescence method and immunofluorescence method respectively. Using immunofluorescence method as a reference system, the precision, correctness, correlation and consistency of electrochemiluminescence method were analyzed respectively. Results: The coefficient of variation of the precision of PCT detected by electrochemical immunofluorescence analyzer was less than 10%, which was consistent with the manufacturers statement. The mean value of electrochemical immunofluorescence analyzer was 16.34ng/ml, the mean value of immunofluorescence analyzer was 15.55ng/ml, the percentage bias was -11.05%, and the standard deviation of the percentage bias was 14.87%. The percent bias confidence interval ranged from -1.53% to -20.57%, covering the stated percent bias of ±15%. The data detected by electrochemiluminescence and immunofluorescence methods were significantly correlated at the level of P<0.01 (y=0.861x+0372, R2=0.960). The Kappa test was used to analyze the consistency between the data detected by electrochemiluminescence and immunofluorescence, and the Kappa value was 0.92. Conclusion: Electrochemical immunofluorescence analyzer has good precision, less bias with immunofluorescence detection system, and good correlation and consistency. In clinical practice, appropriate detection methods can be selected according to specific conditions.

Electrochemiluminescence; Immunofluorescence; Procalcitonin; Performance