| Abstract |
Objective To explore the effect of specimen hemolysis on the accuracy of biochemical test results. Methods: 70 people who were admitted to our hospital for health checkup from January 2020 to December 2020 were selected as the analysis objects. Blood samples were collected from all subjects in the early morning on an empty stomach, and were equally divided into 2 test tubes after the collection was completed. The blood sample in one of the test tubes was regarded as a normal sample, and the blood sample in the other test tube was artificially shaken and shaken to cause hemolysis. An automatic biochemical analyzer was used to detect relevant indicators in normal and hemolyzed samples, including total bilirubin (TBIL), direct bilirubin (DBLL), serum creatinine (Scr), triacylglycerol (TG), total Cholesterol (TC), glucose (GLU), urea (URE-A), uric acid (UA), alanine aminotransferase (ALT), total protein (TP), albumin (ALB), aspartate aminotransferase Enzyme (AST), creatine kinase (CK), potassium ion (K+), urea nitrogen (BUN), calcium ion (Ca2+), lactate dehydrogenase (LDH). Results: After hemolysis of blood samples, the levels of TBIL, Scr, TG, GLU, URE-A, and Ca2+ were lower than those of normal samples, and the levels of DBLL, TC, ALT, TP, ALB, AST, CK, and K+ were higher than those of normal samples. There was statistical significance (P<0.05); in terms of UA, BUN, LDH, etc., there was no significant difference between normal specimens and hemolyzed specimens (P>0.05). Conclusion: Specimen hemolysis will have a significant adverse effect on the accuracy of biochemical test results, resulting in obvious abnormalities in some indicators. In the actual blood biochemical test, effective and correct intervention methods should be taken in time to reduce the risk of hemolysis of the specimen as much as possible, and provide favorable conditions for improving the accuracy of biochemical test results.
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